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Thermal Limits Determination for Zooplankton Using a Heat Block
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JoVE Journal Biology
Thermal Limits Determination for Zooplankton Using a Heat Block

Thermal Limits Determination for Zooplankton Using a Heat Block

DOI:
10.3791/64762-v

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07:16 min

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November 18, 2022

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, ,
1Biology Department,Swarthmore College

Chapters

  • 00:04Introduction
  • 00:46Fabrication of the Heat Block
  • 01:45Determination of Thermal Gradient Settings
  • 02:47Thermal Exposure and Live-Dead Enumeration
  • 05:30Computation of LT50
  • 06:10Results: Survivorship and Upper Thermal Limits of Larval Sand Dollars
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol illustrates the use of commercially available components to generate a stable and linear thermal gradient. Such gradient can then be used to determine the upper thermal limit of planktonic organisms, particularly invertebrate larvae.

Tags

Keywords: Thermal LimitsZooplanktonHeat BlockGlobal WarmingCritical TemperatureAcclimationOntogenyMarine OrganismsPlanktonTemperature GradientWater BathTemperature ControllerRheostatAluminum BlockThermal PasteTygon TubingThermostat ProbeMicro-centrifuge TubesThermocoupleK-type Electrode

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