A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 00:47Preparations
- 01:59Inside the Biological Safety Cabinet
- 04:16Working with Adherent Cells
- 06:13Checking and Storing Cells
- 07:19Working with Suspension Cells
- 08:52Cell Incubation
- 09:05Liquid Waste Collection
- 09:29Clean Up
- 10:38Organization
- 11:13Representative Results
- 12:42Conclusion
This protocol presents essential cell culture techniques and practices to be used in the research cell culture laboratory to avoid contamination by fungi and bacteria. Within the category of bacteria, special emphasis will be placed on preventing mycoplasma contamination.
