Intubation, Central Venous Catheter, and Arterial Line Placement in Swine for Translational Research in Abdominal Transplantation Surgery

Summary

To obtain the best possible results, surgeons performing translational research experiments should be proficient at intubation and vascular line placement. This paper describes the techniques used by the Toronto Organ Preservation Laboratory to perform these procedures.

Abstract

Translational surgical research models in swine are crucial for developing safe preclinical protocols. However, the success of the experimental surgeries does not solely rely on the research team's surgical skills; perioperative care and management procedures, like intubation, central venous line, and arterial line placement, are necessary and of the utmost importance for favorable experiment results. As it is uncommon for research teams to have anesthesiologists or any other staff other than the surgical team, the surgical team involved in translational research must acquire and/or develop the skills to perform the perioperative care. The purpose of this paper is to show the techniques of intubation, central venous catheter, and arterial line placement used and perfected at the Toronto Organ Preservation Laboratory over the last 10 years, to be used as a reference for future researchers joining either this team or any other lab performing translational research protocols in swine and/or abdominal transplantation.

Introduction

Swine experimental models are often used in studying human diseases because of their similar anatomical and physiological properties. These models are crucial in the development of safe pre-clinical protocols, but they are also subject to legal and ethical restrictions¹. The use of swine for research must be done under the best possible conditions to avoid unnecessary loss of animals and suffering due to anesthesia complications unrelated to the research project.

Pre-operative techniques and skills such as intubation, central venous line placement, and arterial line placement are essential for achieving successful and reproducible results.

Every animal undergoing general anesthesia for a surgical procedure must be intubated to maintain an open airway, allowing assisted ventilation and avoiding broncho aspiration². The most common positions for intubation in swine are dorsal, lateral, and sternal recumbency^3,4. Sternal recumbency tends to be easier for personnel trained in human intubation³, which is the case at this research facility.

Good vascular access is essential for the administration of fluids, medications, and sampling during and after surgery. The use of vasopressors is common during abdominal transplant surgeries due to the hemodynamic instability resulting from the ischemia-reperfusion injury. Infusing vasopressors through a peripheral line can cause local tissue injury due to the vasoconstrictive effects⁵. A central venous line placement allows for infusing large amounts of fluids and vasoppresors. We prefer a guidewire-assisted percutaneous technique for central line placement since it minimizes damage to the soft tissue and vessels⁶.

Hemodynamic stability of the animal during surgery is required, and blood pressure is the parameter most typically monitored for this purpose⁷. An arterial line allows for a continuous blood pressure measurement, which is more accurate than traditional non-invasive measurement⁸ as non-invasive techniques underestimate values during hypertension and overestimate them during hypotension^7,8. An accurate reading of blood pressure during these experiments is fundamental to be able to control the amount of fluids and vasopressors that must be administered to the pig.

The Toronto Organ Preservation Laboratory has been using porcine models for more than 10 years and has standardized these procedures throughout the years with excellent outcomes. Although other approaches can be found in the literature for the same procedures, the goal of this paper is to present the techniques developed and perfected over the years at our facility.

Protocol

All animals used for this study received humane care in accordance with the "Principles of Laboratory Animal Care" formulated by the National Society for Medical Research and the "Guide for the Care of Laboratory Animals" published by the National Institutes of Health, Ontario, Canada. All studies were approved by the Animal Care Committee of the Toronto General Research Institute. In this study, 11-12-week-old male Yorkshire pigs weighing 30-40 kg were used.

1. Intubation

1. House the pigs for at least 5 days before the procedure to allow them to adjust to the environment and reduce overall stress levels
2. Fast the pig for 6 h before anesthesia.
3. Anesthetize the pig with an intramuscular (IM) injection of ketamine (20 mg/kg) as a dissociative anesthetic, midazolam (0.15 mg/kg) for sedation, and atropine (0.04 mg/kg) to reduce salivation and bronchial secretions, in the housing facility.
4. Give O₂ and isoflurane at 5% with the help of a mask once the pig has fallen asleep for at least 5 min.
5. Move the pig into a transportation cart and transfer to the operating room (OR).
6. Place the pig in a supine position on the OR table.
7. Place the ventilation mask with 2 L/min O₂ and 5% isoflurane.
   NOTE: Make sure the pig has inspiratory effort.
8. Position the oxygen saturation probe on the pigs' ear.
   NOTE: The probe can also be placed on the tongue, tail, dewclaw³, lips, or inguinal fold.
9. Ask an assistant to place the electrocardiogram electrodes and blood pressure cuff.
   NOTE: Electrodes are placed in the right axillary fold, left axillary fold, and left inguinal fold. The blood pressure cuff can be placed either in the foreleg or hindleg.
10. Check for relaxation of the jaw after 4-5 min of ventilation with isoflurane at 5%.
    NOTE: if resistance is noted, continue ventilation with isoflurane for an additional 3 min or until the jaw is relaxed.
11. Ask the assistant to hold the mandible up, maxilla down, and tongue out of the way.
12. Insert a laryngoscope with a straight miller blade down the midline and lift the epiglottis.
    NOTE: Use an endotracheal tube to push down the soft palate and allow the epiglottis to pop up (Figure 1).
13. Visualize the vocal cords and spray them two times with lidocaine non-aerosol spray (10 mg/metered dose) to prevent spasms during intubation.
14. Remove the laryngoscope and replace the oxygen mask for 30 s.
15. Remove the oxygen mask, insert the Miller straight blade down the midline, and lift the epiglottis. Insert a 7.0 mm endotracheal tube beyond the vocal cords.
    NOTE: If there is resistance or spasm of the vocal cords, repeat steps 1.13 and 1.14.
16. Hold the endotracheal tube in place and slide out the laryngoscope and stylet.
17. Inflate the endotracheal tube cuff with 5-8 cc of air and connect it to a capnometry sensor to ensure the correct placement of the tube.
    NOTE: Other methods to ensure proper placement include looking for chest drives, condensation in the endotracheal tube, and listening to bilateral breath sounds.
18. Turn on the ventilator and adjust to 15-20 breaths per minute, isoflurane to 2%-2.5%, tidal volume to 10-15 mL/kg of bodyweight, and peak inspiratory pressure between 18-20 cmH₂O^9,10,11.
19. Fix the endotracheal tube with tape.
20. Apply eye lube to prevent dryness and ulceration during the procedure.
21. Ensure proper anesthetization by assessing the jaw tone and adjusting isoflurane accordingly. Rigid mandibular muscles indicate a light level of anesthesia³.

2. Central venous catheter placement

NOTE: The choice of catheter depends on the type of model being used. For survival models, a catheter that can be tunneled at the end of the procedure is used. For terminal models, a simpler model (refer to Table of Materials) is used.

1. After intubation, with the pig in the supine position, disinfect both sides of the neck with either iodine or chlorhexidine solution.
2. Prepare the catheter by flushing it with 5 to 10 mL of saline and ensure it does not have air inside. Place the dilator through the catheter.
3. Inspect and test the guidewire to confirm proper aspects and function.
   NOTE: The guidewire must move freely inside the sheath and must not have any defects or kinks. If any alteration is noted, replace the guidewire.
4. Identify the following three landmarks on the pig's neck: (1) the caudal ramus of the mandible, (2) the cranial manubrium, and (3) the cranial point of the right shoulder formed by the greater tubercle of the humerus⁶ (Figure 2).
5. Insert the finder needle attached to a 5 mL syringe in the center of the triangle formed by the three landmarks at a 45° angle from the skin and directed toward the caudal side, while slightly pulling back on the syringe embolus.
6. Once the jugular vein is found, remove the syringe from the finder needle and stabilize the needle while placing the thumb over the needle hub. Ensure the finder needle is secured in place.
   NOTE: If blood coming from the needle is suspected to be arterial, remove the needle immediately and apply pressure for 5 min.
7. Feed the guidewire into the finder needle and advance into the external jugular vein (Seldinger's technique¹²). Minimal to no resistance should be met. Remove the finder needle and keep the guidewire in place.
   NOTE: Use an ECG trace to make sure the guidewire does not go in too deep. If alterations in cardiac rhythm are noted, remove the guidewire slightly.
8. Insert the tip of an 11-blade into the puncture site and create an incision (around 0.5 cm) for the dilator and catheter.
9. Feed the dilator and catheter over the guidewire to dilate the skin and the subcutaneous tissue. Once in place, remove the dilator from the catheter.
10. Aspirate from the catheter lumen to ensure proper flow and flush with 5-10 mL of saline.
11. Suture the catheter into the skin with a 0 silk suture and ensure the catheter is secure¹³.

3. Arterial line placement

NOTE: Carotid placement of the arterial line is preferred since it provides a more direct route to the heart, and the vessels of the neck are larger than the femoral vessels¹¹. Preferably, the arterial line should be placed on the contralateral side of the central venous catheter and under direct vision; if this is not possible, it can be placed on the same side as the venous catheter.

1. Make a vertical incision of 5-7 cm medial to the trachea using a cautery pen (Figure 3).
2. Dissect the subcutaneous tissue and the plane between the cutaneous facii and colli muscles¹¹.
3. Dissect the facial plane between the trachea and the sternomastoideus muscle to localize and open the carotid sheath¹¹ (Figure 4).
4. Use blunt dissection to isolate the common carotid artery (Figure 5).
5. Place two 2-0 silk ties around the carotid artery for vascular control and tie the distal one (Figure 6).
6. Place the right angle forceps under the artery.
7. Ask the assistant to hold the proximal tie and insert the angiocath needle tip into the carotid artery at a 45° angle. Once a flash of blood is seen, advance the needle slightly at a shallow angle and then advance the catheter only into the artery.
8. Remove the needle while keeping the catheter in place.
9. Attach the catheter to the arterial line, aspirate, and flush with 5-10 mL of saline to ensure there is no resistance.
10. Place a tie around the catheter and artery to secure the catheter.

4. After surgery care (survival model)

1. Tunnel the venous line catheter and fix it to the neck of the pig.
2. Once the animal is hemodynamically stable and can be weaned off vasopressors, remove the arterial line catheter, tie the distal side of the carotid artery, and verify hemostasis. Close the wound with an uninterrupted suture.
3. Move the pig to the transportation cart and position it in a sternal position.
4. Turn off isoflurane and monitor the pig until signs of anesthesia recovery are present (movements and breathing against the ventilator).
5. Turn off the ventilator once effective spontaneous respirations are noted, but keep the O₂ supply at 2 L/min.
6. Turn off the O₂ supply after 10-15 min. The pig should be able to maintain a saturation of at least 94% on its own. If that is not the case, turn on the O₂ again and reassess in another 5-10 min.
7. Move the pig back to its pen once it maintains O₂ saturation.
   NOTE: The pig should be moved back to its pen with the endotracheal tube in place.
8. Remove the endotracheal tube once signs of swallowing and/or chewing are evident (between 2-4 h after the end of surgery).
9. Monitor the pig in its pen and never leave it alone. Once the pig is extubated and can maintain a sternal position, it can be left unattended.

Results

Pig monitoring during surgery is indispensable, and the normal parameters expected during surgery are shown in Table 1^3,14. The approximate duration needed to perform each procedure is shown in Table 2.

As mentioned previously, this lab has been performing these procedures for the last 10 years. Over this period, 595 liver experiments, 277 kidney experiments, and 100 pancreas experiments have been carr...

Discussion

Every research center must create its own protocols and guidelines for translational research models; however, some basic rules are to be followed to guarantee successful results.

First, the pig must be submitted to a physical examination as soon as it arrives at the housing facility. A total of 5-7 days of conditioning is necessary before the procedure to reduce stress levels and allow the pig to recover the weight lost during transportation³. It is important to note t...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
0.9% Sodium Chloride Irrigation, USP	Baxter	JF7124	Saline
Angiocath 16 GA 1.88 , 1.7 x 48 mm	BD	381157
Atropine sulfate (8 mg/20 mL - 0.4 mg/mL)	West-Ward	17733	20 mL vial
Cook TPN Single Lumen Cathether Set	Cook medical	G08132	with 10 Fr peel away introducer set
Hickman 9.6 F single-lumen CV cathether	Bard	600560
Laryngoscope	Heine
Midazolam (5 mg/mL)	Sandoz	46237968	10 mL vial
Miller blade 4	Heine		185 mm blade length
Narketan (Ketamine - 100 mg/mL)	Vetoquinal	8-00223	50 mL vial
Nasal tracheal tube cuffed  7.0 mm I.D.	Covidien	86450
Optixcare eye lube for dogs and cats	Aventix	5914304	20 g
Percutaneous Sheath introducer set with integral hemostasis valve/side port for use -7.5 Fr. Catheters	Arrow	SI-09880	8.5 Fr, 10 cm, 0.035 inch dia. Spring wire guide
Universal Electrosurgical Pad: Split with cord	3M	9165
Valleylab Rocker Switch Pencil Holster	Covidien	E2515H
Xylocaine 10% Spray	AstraZeneca	73050036	lidocaine (10 mg/metered dose)