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07:55 min
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February 10, 2023
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Presented here is a protocol to initiate, maintain, and analyze mouse hematopoietic stem cell cultures using ex vivo polyvinyl alcohol-based expansion, as well as methods to genetically manipulate them by lentiviral transduction and electroporation.
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Cite this Article
Khoo, H. M., Meaker, G. A., Wilkinson, A. C. Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures. J. Vis. Exp. (192), e64791, doi:10.3791/64791 (2023).
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