Modified Heterotopic Abdominal Heart Transplantation and a Novel Aortic Regurgitation Model in Rats

Shigeto Tsuji; Shogo Shimada; Minoru Ono

doi:10.3791/64813

Modified Heterotopic Abdominal Heart Transplantation and a Novel Aortic Regurgitation Model in Rats

JoVE Journal
Medicine
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JoVE Journal Medicine

Modified Heterotopic Abdominal Heart Transplantation and a Novel Aortic Regurgitation Model in Rats

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13:32 min

June 2, 2023

DOI: 10.3791/64813-v

Shigeto Tsuji¹, Shogo Shimada¹, Minoru Ono¹

¹Department of Cardiac Surgery,The University of Tokyo

Overview

This study presents a reproducible technique for heterotopic abdominal heart transplantation in rats, designed for beginners. It also introduces a novel model of aortic regurgitation through this transplantation method.

Key Study Components

Area of Science

Cardiovascular surgery
Transplantation techniques
Animal models in research

Background

Heterotopic abdominal heart transplantation is challenging for beginners.
Success rates can vary significantly among inexperienced practitioners.
Creating reliable animal models is crucial for cardiovascular research.
Aortic regurgitation models are important for studying heart function.

Purpose of Study

To demonstrate a beginner-friendly technique for heart transplantation in rats.
To establish a new model for studying aortic regurgitation.
To enhance the accessibility of complex surgical procedures for researchers.

Methods Used

Heterotopic abdominal heart transplantation technique explained.
Donor heart harvesting procedure detailed.
Guidewire method for damaging the donor's aortic valve.
Reproducibility of the technique assessed.

Main Results

A successful method for heterotopic abdominal heart transplantation was established.
The aortic regurgitation model was effectively created.
Beginners were able to replicate the procedure with high success rates.
The study provides a valuable resource for future cardiovascular research.

Conclusions

The study offers a reliable technique for heart transplantation in rats.
The novel aortic regurgitation model can aid in further research.
Accessibility of complex surgical techniques is improved for researchers.

Frequently Asked Questions

What is heterotopic abdominal heart transplantation?

It is a surgical technique where a donor heart is transplanted into the abdomen of a recipient, allowing for functional assessment of the heart.

How does the aortic regurgitation model work?

The model is created by damaging the donor's aortic valve after harvesting, simulating aortic regurgitation in the recipient.

Is this technique suitable for beginners?

Yes, the study demonstrates a reproducible method that beginners can learn and perform successfully.

What are the implications of this research?

This research provides a foundation for studying heart function and disease in a controlled environment using animal models.

Can this technique be applied to other species?

While this study focuses on rats, the principles may be adapted for use in other animal models with appropriate modifications.

What resources are available for learning this technique?

The full transcript and video resources are available for researchers to gain insights into the procedure.

This study demonstrates a reproducible heterotopic abdominal heart transplantation technique in rats that beginners can learn and perform. Additionally, a novel aortic regurgitation model in rats is generated by performing heterotopic abdominal heart transplantation and damaging the donor's aortic valve using a guidewire after harvesting.

Modified heterotopic abdominal heart transplantation and a novel aortic regurgitation model in rats.Introduction. Although some experts can perform heterotopic abdominal heart transplantation in a very short time, it is difficult for beginners to achieve a high success rate. We demonstrate a reproducible method which beginners can perform with ease.

Additionally, we demonstrate the a AR model by using heterotropic abdominal heart transplantation and damaging the donor's aortic valve by a guidewire after harvesting the donor heart.Protocol. First, we explain heterotopic abdominal heart transplantation in rats. Donor heart harvest.

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