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Journal / Biochemistry / Sondando a estrutura do RNA com perfil mutacional …
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Biochemistry

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JoVE Journal Biochemistry
Sondando a estrutura do RNA com perfil mutacional de sulfato de dimetilo com sequenciamento in vitro e em células
Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
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Journal (Biochemistry)
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Sondando a estrutura do RNA com perfil mutacional de sulfato de dimetilo com sequenciamento in vitro e em células

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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O protocolo fornece instruções para modificar o RNA com sulfato de dimetilo para experimentos de perfil mutacional. Inclui sondagem in vitro e in vivo com dois métodos alternativos de preparação de bibliotecas.

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Bioquímica Edição 190
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