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Realtidskvantificering af virkningerne af IS200/IS605 familieassocieret TnpB på transponeringsaktivitet
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JoVE Journal Biology
Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Realtidskvantificering af virkningerne af IS200/IS605 familieassocieret TnpB på transponeringsaktivitet

DOI:
10.3791/64825-v

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04:04 min

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January 20, 2023

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, ,
1Department of Physics and Astronomy,University of California, 2Microbiology Program,University of California, 3Biophysics Program,University of California

Chapters

  • 00:05Introduction
  • 00:44Slide Preparation
  • 01:59Timelapse Fluorescence Microscopy
  • 02:25Results: Visualizing Transposon Activity in Live E. coli Cells
  • 03:30Conclusion

Summary

Automatic Translation

Automatic Translation

En protokol er skitseret til at udføre live realtidsbilleddannelse for at kvantificere, hvordan tilbehørsproteinet TnpB påvirker transponeringsdynamikken i individuelle levende Escherichia coli-celler .

Tags

Real-time QuantificationIS200/IS605 FamilyTnpBTransposon ActivitySingle-cell AnalysisLive Cell ImagingFluorescence MicroscopyExcision DynamicsTransposaseM63 Agarose PadBacterial CultureEpoxy SealTime-lapse Imaging

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