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Gelatin Methacryloyl Granular Hydrogel Scaffolds
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Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting

Article DOI: 10.3791/64829-v • 10:36 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 2, 1,2
1Department of Chemical Engineering, The Pennsylvania State University, 2Department of Biomedical Engineering, The Pennsylvania State University

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This article describes protocols for high-throughput gelatin methacryloyl microgel fabrication using microfluidic devices, converting microgels to resuspendable powder (micro-aerogels), the chemical assembly of microgels to form granular hydrogel scaffolds, and developing granular hydrogel bioinks with preserved microporosity for 3D bioprinting.

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Gelatin Methacryloyl Granular Hydrogel Scaffolds High-throughput Microgel Fabrication Lyophilization Chemical Assembly 3D Bioprinting ECM Numeric Hydrogel Microparticles Scaffold Fabrication Purification Photo Assembly Cell-responsive Biomaterial Biodegradable Moieties Cell Infiltration Building Block Size 3D Bioprinting Of Thick Tissues And Organs Regenerative Engineering LAP Photoinitiator Solution GelMA Clear Solution Oil Phase Preparation Biocompatible Surfactant Solution
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