Journal
/
Biology
/
The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation

The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation

DOI:
10.3791/64867-v

•

05:38 min

•

June 23, 2023

•

, , , ,
1Gynecology Research Unit, Institut de Recherche Expérimentale et Clinique,Université Catholique de Louvain, 2Gynecology Department,Cliniques Universitaires Saint-Luc

Chapters

  • 00:00Introduction
  • 00:57Preparing the Eggs for Incubation and Opening the Eggshell on Day 3 of Chick Embryo Development (ED)
  • 02:38Grafting Frozen-Thawed Human Ovarian Tissue to the CAM and Harvesting the Grafts
  • 03:33Results: Development of a CAM Xenografting Model for Human Ovarian Tissue
  • 04:37Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol for developing a chick chorioallantoic membrane (CAM) xenografting model for human ovarian tissue and demonstrate the effectiveness of the technique, the graft revascularization time frame, and the tissue viability across a 6 day grafting period.

Tags

Keywords: Chick Chorioallantoic Membrane (CAM) ModelNon-embryonated EggsEgg CandlerEgg ManipulationCAM VascularizationFollicle LossEther-extracted Lens Paper

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64867?article_type=v

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
check_url/64867?article_type=v

Computer-Generated Animal Model Stimuli
check_url/64867?article_type=v

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
check_url/64867?article_type=v

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
check_url/64867?article_type=v

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
check_url/64867?article_type=v

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
check_url/64867?article_type=v

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
check_url/64867?article_type=v

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
check_url/64867?article_type=v

Isolation of Embryonic Ventricular Endothelial Cells
check_url/64867?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Read Article