Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

Patient drive organoid models genetically and functionally represent their parent tumors and thus are reliable models for studying novel therapeutics and complex biologic processes in ovarian cancer. By recapitulating clonal heterogeneity, the tumor microenvironment, and in vitro cell to cell interactions, these models overcome the limitations of cancer cell lines and patient derived xenografts. Patient derived organoids offer long-term expansion and storage capabilities and mimic tumor physiology.

As a result, they are ideal models for the study of novel therapeutics and predictive biomarkers. Begin harvesting of the organoids by placing the tumor samples in a 10 centimeter tissue culture dish and mincing the tissue to create a homogenous mixture using a disposable scalpel. Place the homogenous tissue mixture into a dissociation tube using forceps.