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Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
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Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

DOI:
10.3791/64885-v

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05:26 min

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February 03, 2023

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, , , , , , , , , , , ,
1Department of Medical, Oral and Biotechnological Sciences,“G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST),“G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences,University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti,Università degli Studi di Milano, 6Department of Pharmaceutical Sciences,Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases,Centro Cardiologico Monzino IRCCS

Chapters

  • 00:04Introduction
  • 00:45Sample Preparation and Enzymatic Digestion
  • 01:47Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
  • 02:32Sedimentation of Cell Clusters and Cell Seeding
  • 03:16Results: Purification and Characterization of Freshly Isolated HLMVECs
  • 04:30Conclusion

Summary

Automatic Translation

Automatic Translation

Although challenging, the isolation of pulmonary endothelial cells is essential for studies on lung inflammation. The present protocol describes a procedure for the high-yield, high-purity isolation of macrovascular and microvascular endothelial cells.

Tags

Keywords: Endothelial CellsLungBlood VesselsPurificationIsolationMicrovascularMacrovascularCollagenaseCell StrainerCentrifugationCell Culture

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