Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Functional Characterization and Visualization of…
JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology
Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE
Journal (Biology)
Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE
Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
Journal (Biology)
Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
 

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Article DOI: 10.3791/64905-v • 11:58 min • January 6th, 2023
January 6th, 2023 •
1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

Chapters

Summary
Automatic Translation

Organoid-fibroblast co-cultures provide a model to study the in vivo stem cell niche. Here, a protocol for esophageal organoid-fibroblast co-cultures is described. Additionally, whole mount imaging is used to visualize the fibroblast-organoid interaction.

Tags

Functional Characterization Visualization Esophageal Fibroblasts Organoid Co-cultures Progenitor Cells Tissue Homeostasis Fibroblast Behavior Co-culture System Esophageal Progenitor Niche Organoid Clearing Cell Interactions Organoid Morphology Human Esophageal Organoids Cancer-associated Fibroblasts Dissociation Of Cells Viable Organoids Muscularis Externa Removal Esophagus Dissection Tissue Damage Prevention
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter