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JoVE Journal
Bioengineering

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Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
 

Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells

Article DOI: 10.3791/64909-v 10:08 min June 16th, 2023
June 16th, 2023

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Summary

Here, we present light-induced dielectrophoresis as a label-free approach for characterizing heterogeneous cell lines, specifically human mesenchymal stem cells (hMSCs). This paper describes a protocol for using and optimizing a microfluidic device with a photoconductive layer to characterize the electrical behavior of hMSCs without altering their native state.

Tags

Light-induced Dielectrophoresis LiDEP Human Mesenchymal Stem Cells Stem Cell Characterization Virtual Electrode Configuration Microfluidic Device Zuri Rashad Graduate Student Microchannel Preparation Double-sided Tape Scalpel Inlet And Outlet Holes ITO G
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