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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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Developmental Biology
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JoVE Journal Developmental Biology
Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

DOI:
10.3791/65030-v

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09:06 min

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January 20, 2023

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,
1Chan Zuckerberg Biohub, 2Department of Bioengineering and Therapeutic Sciences,University of California San Francisco, 3Programs in Human Genetics and Biological Sciences,University of California San Francisco

Chapters

  • 00:04Introduction
  • 01:01Preparation of Zebrafish Embryos
  • 02:10Preparation of Microinjection
  • 03:51Microinjection
  • 06:02Mounting and Time‐Lapse Live Imaging
  • 07:32Results: Imaging Anti‐Dld‐Atto‐647N Antibody Uptake in the Developing Zebrafish Brain
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

This work develops an antibody uptake assay for imaging intra-lineage Notch/DeltaD signaling in dividing radial glia progenitors of the embryonic zebrafish brain.

Tags

Keywords: Antibody Uptake AssayNotch/Delta EndocytosisAsymmetric DivisionZebrafish Radial Glia ProgenitorsNotch SignalingSulfate DecisionPatterningDevelopmentMetazoansAnti-Dld AntibodyAnti-mouse IgG Atto-647N AntibodyBrain VentriclesEmbryonic DevelopmentMicroinjectionFluorescence Microscopy

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