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Developmental Biology

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JoVE Journal Developmental Biology
Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
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Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Article DOI: 10.3791/65030-v • 09:06 min • January 20th, 2023
January 20th, 2023 •
1,2, 2,3
1Chan Zuckerberg Biohub, 2Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 3Programs in Human Genetics and Biological Sciences, University of California San Francisco

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Summary
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This work develops an antibody uptake assay for imaging intra-lineage Notch/DeltaD signaling in dividing radial glia progenitors of the embryonic zebrafish brain.

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Antibody Uptake Assay Notch/Delta Endocytosis Zebrafish Radial Glia Progenitors Developmental Patterning Zebrafish Forebrain Anti-Dld-Atto-647N Uptake Brain Ventricular Injection Pharmacological Perturbations Genetic Perturbations Developmental Stages Adult Brain Pluripotent Stem Cells Brain Organoids Fertilized Embryos Epifluorescence Microscope White Light Magnification Dead Embryos Glass Pipette RFP Filter Setting Red Fluorescence Egg Water Dechorionate Embryos
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