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Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
JoVE Journal
Biology
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JoVE Journal Biology
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

DOI:
10.3791/65040-v

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10:49 min

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February 03, 2023

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, , , ,
1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),University Medical Center Utrecht, 2Oncode Institute,Hubrecht Institute

Chapters

  • 00:04Introduction
  • 00:39Organoid Derivation
  • 03:41Functional Swelling Assay
  • 04:45Organoid Electroporation
  • 06:36Orthotopic Transplantation of Human Lacrimal Gland Organoids
  • 08:17Results: Establishment, Genetic Modification, Differentiation, Functional Characterization, and Transplantation of Lacrimal Gland Organoids
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to establish, maintain, genetically modify, differentiate, functionally characterize, and transplant lacrimal gland organoids derived from primary mouse and human tissue.

Tags

Keywords: Lacrimal Gland OrganoidsAdult Stem CellRegenerative MedicineDry Eye DiseaseExtracellular MatrixCell CultureMouseHuman

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