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A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

DOI:
10.3791/65044-v

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09:12 min

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February 03, 2023

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, , , ,
1School of Medicine,South China University of Technology, 2Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery,Guangzhou Women and Children’s Medical Center

Chapters

  • 00:04Introduction
  • 00:50Establishment of the Chronic Fibrotic BA Mouse Model
  • 01:45Intraperitoneal Injection of the Mice
  • 02:36Collection of Sample Tissue
  • 03:13Fluorescein Angiography of the Extrahepatic Bile Duct
  • 03:41H&E Staining
  • 04:43CK19 and F4/80 Immunohistochemical Staining
  • 06:00Sirius Red Staining
  • 06:55Results: Effects of Anti‐Ly6G Antibody Treatment in a Mouse Model of Biliary Atresia (BA) Infected with RRV
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments.

Tags

Keywords: Mouse ModelChronic Liver FibrosisBiliary AtresiaRhesus RotavirusAnti-Ly6G AntibodyIntraperitoneal InjectionLiver FunctionFluorescent DyeGallbladderExtrahepatic Bile Ducts

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