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Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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Developmental Biology
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JoVE Journal Developmental Biology
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

DOI:
10.3791/65069-v

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07:44 min

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March 03, 2023

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, , ,
1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences,University of Lisbon

Chapters

  • 00:04Introduction
  • 01:12Fetus Tissue Excision
  • 02:12Fetal Skeletal Muscle Decellularization
  • 03:38Cell Culture in Decellularized Matrices
  • 05:24Results: Fetal Muscle Tissue Protein Analysis
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

In this work, a decellularization protocol was optimized to obtain decellularized matrices of fetal mouse skeletal muscle. C2C12 myoblasts can colonize these matrices, proliferate, and differentiate. This in vitro model can be used to study cell behavior in the context of skeletal muscle diseases such as muscular dystrophies.

Tags

Keywords: 3D Decellularized MatricesFetal Mouse Skeletal MuscleCell CultureLAMA2-CMDCongenital Muscle DystrophyExtracellular MatrixDevelopmental ModelProtocolDecellularizationWashingStorage

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