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Developmental Biology

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JoVE Journal Developmental Biology
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Article DOI: 10.3791/65069-v • 07:44 min • March 3rd, 2023
March 3rd, 2023 •
1, 1, 1, 1
1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences, University of Lisbon

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Summary
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In this work, a decellularization protocol was optimized to obtain decellularized matrices of fetal mouse skeletal muscle. C2C12 myoblasts can colonize these matrices, proliferate, and differentiate. This in vitro model can be used to study cell behavior in the context of skeletal muscle diseases such as muscular dystrophies.

Tags

3D Decellularized Matrices Fetal Mouse Skeletal Muscle Cell Culture Myopathies Muscle Cell Behavior Extracellular Matrix LAMA2-CMD Congenital Muscle Dystrophy Target Therapies Tissue Adaptation Disease Models Sample Collection And Handling Technical Skills Protocol Steps
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