Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Martino Guiotto; Silvia Palombella; Stefania Brambilla; Lee Ann Applegate; Mathis Riehle; Andrew Hart; Wassim Raffoul; Pietro Giovanni di Summa

doi:10.3791/65104

Journal

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Medicine

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Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

JoVE Journal
Medicine

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JoVE Journal Medicine

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

3,169 Views

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06:00 min

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February 03, 2023

DOI:

10.3791/65104-v

DOI:

10.3791/65104-v

•

06:00 min

February 03, 2023

•

3131 Views

Martino Guiotto*^1,4, Silvia Palombella*², Stefania Brambilla², Lee Ann Applegate³, Mathis Riehle⁴, Andrew Hart⁴, Wassim Raffoul¹, Pietro Giovanni di Summa¹

¹Department of Plastic, Reconstructive and Hand Surgery,Centre Hospitalier Universitaire Vaudois (CHUV ²Cell and Tissue Engineering Lab,IRCCS Ospedale Galeazzi Sant’Ambrogio ³Unit of Regenerative Therapy, Plastic, Reconstructive and Hand Surgery, Department of Musculoskeletal Medicine,Lausanne University Hospital ⁴Centre for the Cellular Microenvironment,University of Glasgow

Summary

Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.