Name: Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method (Video) | JoVE Journal
Uploaded: 2023-05-19T14:40:00.000+00:00
Duration: 9 min 12 s
Description: Learn to optimize Tiebangchui processing with highland barley wine using Box-Behnken design and entropy method. This protocol demonstrates a stable, visible approach for toxicity attenuation in pharmaceutical applications.

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This work was financially supported by the National Natural Science Foundation of China (No. 82130113), the China Postdoctoral Science Foundation (No. 2021MD703800), the Science Foundation for Youths of Science &#38; Technology Department of Sichuan Province (No. 2022NSFSC1449), and the &#34;Xinglin Scholars&#34; Research Promotion Program of Chengdu University of Traditional Chinese Medicine (No. BSH2021009).

<ol><li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Study+of+quality+standards+for+Aconitum+pendulum+Busch%5BTitle%5D)+AND+%22Chinese+Traditional+Patent+Medicine%22%5BJournal%5D)">Study of quality standards for Aconitum pendulum Busch.</a> Chinese Traditional Patent Medicine. 40 (9), 2100-2103 (2018).</li>
<li>Liu, X. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Study+on+toxicity+reduction+and+effect+preservation+of+the+compatibility+of+Tibetan+medicine+Aconitum+pendulum+Busch+and+Terminalia+chebula+Retz%5BTitle%5D)+AND+%22Pharmacy+and+Clinics+of+Chinese+Materia+Medica%22%5BJournal%5D)">Study on toxicity reduction and effect preservation of the compatibility of Tibetan medicine Aconitum pendulum Busch and Terminalia chebula Retz.</a> Pharmacy and Clinics of Chinese Materia Medica. 13 (3), 69-72 (2022).</li>
<li>Luo, D. S. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aLuo%2C+DS+%22Chinese+Tibetan+herbs%22">Chinese Tibetan herbs</a>. 63, Ethnic publishing House. Beijing. (2007).</li>
<li>Li, C. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aconitum+pendulum+Busch+and+A.+flavum+Hand-Mazz%3A+A+narrative+review+on+traditional+uses%2C+phytochemistry%2C+bioactivities+and+processing+methods%5BTitle%5D)+AND+%22Journal+of+Ethnopharmacology%22%5BJournal%5D)">Aconitum pendulum Busch and A. flavum Hand-Mazz: A narrative review on traditional uses, phytochemistry, bioactivities and processing methods.</a> Journal of Ethnopharmacology. 292, 115216(2022).</li>
<li>Yu, L. Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traditional+Tibetan+medicine%3A+therapeutic+potential+in+rheumatoid+arthritis%5BTitle%5D)+AND+%22Frontiers+in+Pharmacology%22%5BJournal%5D)">Traditional Tibetan medicine: therapeutic potential in rheumatoid arthritis.</a> Frontiers in Pharmacology. 13, 938915(2022).</li>
<li>Zhang, Y., Fu, X. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((UPLC+Simultaneous+determination+of+six+esteric+alkaloids+components+in+Aconitum+pendulum+Busch%5BTitle%5D)+AND+%22Asia-pacific+Traditional+Medicine%22%5BJournal%5D)">UPLC Simultaneous determination of six esteric alkaloids components in Aconitum pendulum Busch.</a> Asia-pacific Traditional Medicine. 16 (5), 62-65 (2020).</li>
<li>Wang, Y. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Determination+of+alkaloid+content+in+different+medicinal+parts+of+the+folk+medicine.+Aconitum+pendulum+Busch%5BTitle%5D)+AND+%22Chinese+Traditional+Patent+Medicine%22%5BJournal%5D)">Determination of alkaloid content in different medicinal parts of the folk medicine. Aconitum pendulum Busch.</a> Chinese Traditional Patent Medicine. 32 (8), 1390-1393 (2010).</li>
<li>Shao, C. L., Fu, J. L., Fu, S. X., Ma, H. W., Sun, X. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Toxicity+research+and+processing+methods+of+Aconitum+pendulum+Busch%5BTitle%5D)+AND+%22Asia-pacific+Traditional+Medicine%22%5BJournal%5D)">Toxicity research and processing methods of Aconitum pendulum Busch.</a> Asia-pacific Traditional Medicine. 10 (2), 32-34 (2014).</li>
<li>Chan, T. Y. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aconite+poisoning%5BTitle%5D)+AND+%22Clinical+Toxicology%22%5BJournal%5D)">Aconite poisoning.</a> Clinical Toxicology. 47 (4), 279-285 (2009).</li>
<li>Li, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+insight+into+current+advances+on+pharmacology%2C+pharmacokinetics%2C+toxicity+and+detoxification+of+aconitine%5BTitle%5D)+AND+%22Biomedicine+%26+Pharmacotherapy%22%5BJournal%5D)">An insight into current advances on pharmacology, pharmacokinetics, toxicity and detoxification of aconitine.</a> Biomedicine & Pharmacotherapy. 151, 113115(2022).</li>
<li>Zhao, M. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Study+on+identification+and+processing+attenuation+of+Tibetan+drug+Bangna%5BTitle%5D)+AND+%22Southwest+Jiaotong+University%22%5BJournal%5D)">Study on identification and processing attenuation of Tibetan drug Bangna.</a> Southwest Jiaotong University. , (2018).</li>
<li>Tibet Autonomous Region Food and Drug Administration. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Tibetan+herbal+medicine+concoction+specification%22">Tibetan herbal medicine concoction specification</a>. , Tibetan People's Publishing House. Lhasa. 135(2008).</li>
<li>Abd-El-Aziz, N. M., Hifnawy, M. S., El-Ashmawy, A. A., Lotfy, R. A., Younis, I. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+Box-Behnken+design+for+optimization+of+phenolics+extraction+from+Leontodon+hispidulus+in+relation+to+its+antioxidant%2C+anti-inflammatory+and+cytotoxic+activities%5BTitle%5D)+AND+%22Scientific+Reports%22%5BJournal%5D)">Application of Box-Behnken design for optimization of phenolics extraction from Leontodon hispidulus in relation to its antioxidant, anti-inflammatory and cytotoxic activities.</a> Scientific Reports. 12 (1), 8829(2022).</li>
<li>Quan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+processing+technology+of+stir-frying+with+vinegar+of+Curcuma+Longa+Radix+by+orthogonal+design+and+Box-Behnken+design-response+surface+based+on+entropy+method%5BTitle%5D)+AND+%22Chinese+Traditional+and+Herbal+Drugs%22%5BJournal%5D)">Optimization of processing technology of stir-frying with vinegar of Curcuma Longa Radix by orthogonal design and Box-Behnken design-response surface based on entropy method.</a> Chinese Traditional and Herbal Drugs. 49 (8), 1823-1828 (2018).</li>
<li>Jia, F. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Research+on+the+brewing+technology+of+Tibetan+traditional+barley+liquor+based+on+response+surface+method%5BTitle%5D)+AND+%22Food+and+Fermentation+Industries%22%5BJournal%5D)">Research on the brewing technology of Tibetan traditional barley liquor based on response surface method.</a> Food and Fermentation Industries. 45 (22), 171-178 (2019).</li>
<li>Zhao, X. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Determination+of+osthol+in+different+traditional+Chinese+medicines+by+HPLC%5BTitle%5D)+AND+%22Chinese+Journal+of+Pharmaceutics%22%5BJournal%5D)">Determination of osthol in different traditional Chinese medicines by HPLC.</a> Chinese Journal of Pharmaceutics. 19 (5), 154-158 (2021).</li>
<li>Feng, Z. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Processing+methods+and+the+underlying+detoxification+mechanisms+for+toxic+medicinal+materials+used+by+ethnic+minorities+in+China%3A+A+review%5BTitle%5D)+AND+%22Journal+of+Ethnopharmacology%22%5BJournal%5D)">Processing methods and the underlying detoxification mechanisms for toxic medicinal materials used by ethnic minorities in China: A review.</a> Journal of Ethnopharmacology. 305, 116126(2023).</li>
<li>Wang, D. M., Lu, Z. J., Wang, Y. H., Zhang, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applying+grading+methods+of+synthesizing+multiple+guidelines+to+optimizing+alcohol-steam+processing+technology+from+Ploygonatum+odordatum%5BTitle%5D)+AND+%22Journal+of+Zhejiang+A+%26+F+University%22%5BJournal%5D)">Applying grading methods of synthesizing multiple guidelines to optimizing alcohol-steam processing technology from Ploygonatum odordatum.</a> Journal of Zhejiang A & F University. 30 (1), 100-106 (2013).</li>
<li>He, N. L., Bao, M. L., Ba, G. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Study+on+the+best+processing+technology+of+Terminalia+Decoction+soaking+iron%5BTitle%5D)+AND+%22Journal+of+Medicine+%26+Pharmacy+of+Chinese+Minorities%22%5BJournal%5D)">Study on the best processing technology of Terminalia Decoction soaking iron.</a> Journal of Medicine & Pharmacy of Chinese Minorities. 20 (9), 36-38 (2014).</li>
<li>Liu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+processing+technology+for+Saposhnikoviae+Radix+by+Box-Behnken+design-response+surface+methodology%5BTitle%5D)+AND+%22Chinese+Journal+of+Experimental+Traditional+Medical+Formulae%22%5BJournal%5D)">Optimization of processing technology for Saposhnikoviae Radix by Box-Behnken design-response surface methodology.</a> Chinese Journal of Experimental Traditional Medical Formulae. 20 (5), 18-21 (2014).</li>
<li>Ferreira, S. L. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Box-Behnken+design%3A+an+alternative+for+the+optimization+of+analytical+methods%5BTitle%5D)+AND+%22Analytica+Chimica+Acta%22%5BJournal%5D)">Box-Behnken design: an alternative for the optimization of analytical methods.</a> Analytica Chimica Acta. 597 (2), 179-186 (2007).</li>
<li>Dong, R., Lu, Y., Wang, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+process+optimization+of+vinegar+roasting+of+Bupleurum+chinense+by+entropy+weight+method+combined+with+Box-Behnken+response+surface+method+and+its+protective+effect+on+mice+liver+injury%5BTitle%5D)+AND+%22Science+and+Technology+of+Food+Industry%22%5BJournal%5D)">The process optimization of vinegar roasting of Bupleurum chinense by entropy weight method combined with Box-Behnken response surface method and its protective effect on mice liver injury.</a> Science and Technology of Food Industry. 42 (23), 209-217 (2021).</li>
<li>Li, W. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analysis+on+the+times+of+Polygonati+Rhizoma+steamed+by+multiple+times+based+on+entropy+weight+and+gray+relative+analysis+method%5BTitle%5D)+AND+%22China+Journal+of+Traditional+Chinese+Medicine+and+Pharmacy%22%5BJournal%5D)">Analysis on the times of Polygonati Rhizoma steamed by multiple times based on entropy weight and gray relative analysis method.</a> China Journal of Traditional Chinese Medicine and Pharmacy. 36 (11), 6764-6769 (2021).</li>
<li>Huang, B. J., Liu, X. T., Mao, Y. M., Qi, B., Liu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Response+surface+methodology+combined+with+analytic+hierarchy+process+to+optimize+the+processing+technology+of+Custutae+semen+with+wine%5BTitle%5D)+AND+%22Lishizhen+Medicine+and+Materia+Medica+Research%22%5BJournal%5D)">Response surface methodology combined with analytic hierarchy process to optimize the processing technology of Custutae semen with wine.</a> Lishizhen Medicine and Materia Medica Research. 33 (8), 1890-1894 (2022).</li>
<li>Wang, J., Meng, X. H., Chai, T., Yang, J. L., Shi, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Diterpenoid+alkaloids+and+one+lignan+from+the+roots+of+Aconitum+pendulum+Busch%5BTitle%5D)+AND+%22Natural+Products+and+Bioprospecting%22%5BJournal%5D)">Diterpenoid alkaloids and one lignan from the roots of Aconitum pendulum Busch.</a> Natural Products and Bioprospecting. 9 (6), 419-423 (2019).</li>
<li>Xie, H. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolomics+study+of+aconitine+and+benzoylaconine+induced+reproductive+toxicity+in+Be+Wo+cell%5BTitle%5D)+AND+%22Chinese+Journal+of+Analytical+Chemistry%22%5BJournal%5D)">Metabolomics study of aconitine and benzoylaconine induced reproductive toxicity in Be Wo cell.</a> Chinese Journal of Analytical Chemistry. 43 (12), 1808-1813 (2015).</li>
<li>Han, Y. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+extraction+process+for+Yangyin+Runmu+granules+by+Box-Behnken+design+based+on+entropy+weight+method-analytic+hierarchy+process+method%5BTitle%5D)+AND+%22Chinese+Journal+of+Modern+Applied+Pharmacy%22%5BJournal%5D)">Optimization of extraction process for Yangyin Runmu granules by Box-Behnken design based on entropy weight method-analytic hierarchy process method.</a> Chinese Journal of Modern Applied Pharmacy. 39 (7), 896-903 (2022).</li>
<li>Chen, F. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+the+baked+drying+technology+of+Clinamomi+Ramulus+based+on+CRITIC+combined+with+Box-Behnken+response+surface+method%5BTitle%5D)+AND+%22Journal+of+Chinese+Medicinal+Materials%22%5BJournal%5D)">Optimization of the baked drying technology of Clinamomi Ramulus based on CRITIC combined with Box-Behnken response surface method.</a> Journal of Chinese Medicinal Materials. 2022 (8), 1838-1842 (2022).</li>
<li>Pan, Y. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+stir-baking+process+of+Coix+lacryma-Jobi+Var.Mayuen+Kernel+by+Box-Behnken+response+surface+methodology%5BTitle%5D)+AND+%22Shandong+Chemical+Industry%22%5BJournal%5D)">Optimization of stir-baking process of Coix lacryma-Jobi Var.Mayuen Kernel by Box-Behnken response surface methodology.</a> Shandong Chemical Industry. 51 (14), 73-75 (2022).</li>
</ol>

The authors have no conflicts of interest to disclose.

As a commonly used Tibetan medicine with toxic effects, the toxicity-attenuating effect of processing is extremely important for TBC&#39;s clinical application25. In this study, the processing technology of TBC processed with highland barley wine was optimized. By reviewing the main active ingredients and relating the pharmacological effects of TBC, we found that TBC alkaloids have anti-inflammatory and analgesic effects and can be used to treat rheumatoid arthritis. In this study, a moderate toxi...

Tiebangchui (TBC), the dried root of Aconitum pendulum Busch, is a well-known Tibetan medicine and was initially recorded in the classic Tibetan medical book &#34;Four Medical Tantra&#34;1,2. According to &#34;Drug Standards of the Ministry of Health of the People&#39;s Republic of China (Tibetan Medicine)&#34;, TBC is effective in expelling cold, relieving pain, dispelling wind, and calming shock, and is commonly used to treat rheumatoid arthritis in clinics3,4,5.
TBC mainly contains alkaloids, including highly toxic diester-diterpenoid alkaloids (DDAs), and the moderately toxic monoester-diterpenoid alkaloids (MDAs)6,7,8. These chemical components are active ingredients with medicinal effects but are toxic. One of the most famous active and toxic ingredients, aconitine, causes poisoning when it exceeds 1 mg9. Therefore, improper or excessive use of TBC might result in poisoning and even death, and the toxicity attenuation and efficacy reservation of TBC is crucial for its safe clinical application10,11.
Processing is an effective method for detoxifying TBC. According to ancient Tibetan medicine books, processing with highland barley wine is an efficient way to attenuate toxicity and preserve the efficacy of TBC. TBC is soaked in highland barley wine, stored for one night, dried, and added to medicines12. However, the specific processing technology and potential influencing factors are rarely reported, and the traditional processing process often relies on experience and lacks standardized methods. Hence, modern scientific and technological methods for optimizing and standardizing the processing process are needed.
The Box-Behnken design method is used in investigating interactions among different factors and their influence on comprehensive scoring through quadratic polynomial fitting. This design allows the intuitive observation of optimal conditions and has been widely used in the field of pharmacy13. For example, the Box-Behnken design method, based on the entropy method, successfully optimized the processing technology of stir-frying with vinegar of Curcuma Longa Radix14. In this study, the Box-Behnken response surface experimental design combined with the entropy method was used in optimizing the processing technology of TBC processed with highland barley wine. The optimized processing technology is expected to ensure quality control and safe clinical use.

<table><tbody><tr><td>Aconitine</td><td>Chengdu Push Biotechnology Co.,Ltd</td><td>PS000905</td><td></td></tr><tr><td>3-Acetylaconitine</td><td>Chengdu Push Biotechnology Co.,Ltd</td><td>PS010552</td><td></td></tr><tr><td>3-Deoxyaconitine</td><td>Chengdu Push Biotechnology Co.,Ltd</td><td>PS011258</td><td></td></tr><tr><td>Benzoylaconine</td><td>Chengdu Push Biotechnology Co.,Ltd</td><td>PS010300</td><td></td></tr><tr><td>Circulating water vacuum pump</td><td>Gongyi City Yuhua Instrument Co., Ltd</td><td>SHZ-DIII</td><td></td></tr><tr><td>Design-Expert&amp;nbsp;</td><td>State-East Corporation</td><td>8.0.6</td><td></td></tr><tr><td>Electric constant temperature drying oven</td><td>Shanghai Yuejin Medical Equipment Co., Ltd</td><td>101-3-BS</td><td></td></tr><tr><td>Electronic analytical balance</td><td>Shanghai Liangping Instruments Co., Ltd.</td><td>FA1004</td><td></td></tr><tr><td>High performance liquid chromatography</td><td>Shimadzu Enterprise Management (China) Co., Ltd</td><td>shimadzu 2030</td><td></td></tr><tr><td>Highland barley rice</td><td>Kangding City, Ganzi Tibetan Autonomous Prefecture, Sichuan Province</td><td>20221015</td><td></td></tr><tr><td>Millipore filter</td><td>Tianjin Jinteng Experimental Equipment Co., Ltd</td><td>&amp;phi;13 0.22 Nylon66</td><td></td></tr><tr><td>Rotary evaporator</td><td>Shanghai Yarong Biochemical Instrument Factory</td><td>RE-2000A</td><td></td></tr><tr><td>Starter of liquor-making</td><td>Angel Yeast CO., Ltd</td><td>BJ22-104</td><td></td></tr><tr><td>Ultra pure water systemic</td><td>Merck Millipore Ltd.</td><td>Milli-Q</td><td></td></tr><tr><td>Ultrasonic cleansing machine</td><td>Ningbo Xinyi Ultrasonic Equipment Co., Ltd</td><td>SB-8200 DTS</td><td></td></tr></tbody></table>

optimization of processing of tiebangchui with highland barley wine based on the box-behnken design combined with the entropy method

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method

The processing of toxic ethnomedicines is of great significance for their safe clinical application. Thus, the limitations of traditional processing should be addressed, and the processing method of ethnomedicines should be standardized using modern research methods. In this study, the processing technology of a commonly used Tibetan medicine Tiebangchui (TBC), the dried root of Aconitum pendulum&#160;Busch, processed with highland barley wine was optimized. Diester-diterpenoid alkaloid (DDA) (aconitine, 3-deoxyaconitine, 3-acetylaconitine) and monoester-diterpenoid alkaloid (MDA) (benzoylaconine) content were used as evaluation indicators, and the weight coefficient of each evaluation index was determined by the entropy method.
The single factor test and Box-Behnken design were used in investigating the influence of the ratio between highland barley wine and TBC, slice thickness of TBC, and processing time. Comprehensive scoring was performed according to the objective weight of each index determined by the entropy method. The optimal processing conditions of TBC with highland barley wine were as follows: the amount of highland barley wine is five times that of TBC, a soaking time of 24 h, and a TBC thickness of 1.5 cm. The results showed that the relative standard deviation between the verification test and predicted value was less than 2.55% and the optimized processing technology of TBC processed with highland barley wine is simple, feasible, and stable, and so can provide a reference for industrial production.

In this study, the precision, stability, repeatability, and sample recovery of TBC indicated that the method is feasible. The four index components in TBC had a good linear relationship within a specific concentration range. Typical chromatograms are shown in Figure 1. The precision test results (Table 8) showed that the relative standard deviation (RSD) of the peak areas were 2.56%, 1.49%, and 2.03% for benzoylaconine, aconitine, and 3-deoxyaconitine, respectively, and 0.21...

The present protocol describes an efficient method for optimization of the processing technology of Tiebangchui processed with highland barley wine based on a Box-Behnken design response surface combined with the entropy method.

The processing of toxic ethnomedicines is of great significance for their safe clinical application. Thus, the limitations of traditional processing ...

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Learn to optimize Tiebangchui processing with highland barley wine using Box-Behnken design and entropy method. This protocol demonstrates a stable, visible approach for toxicity attenuation in pharmaceutical applications.

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method (Video) | JoVE Journal

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method

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