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Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

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05:59 min

May 19, 2023

DOI:

05:59 min
May 19, 2023

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Transcript

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Our research focuses on how mitochondria sense and adapt to metabolic stressors. One key metabolic stressor is hypoxia, which occurs when cells are starved of oxygen. We are developing better assays to study mitochondrial function in low-oxygen environments.

Higher resolution mass spectrometry was employed to study cell and tissue metabolite levels. We used stable isotope tracing studies to track the fate of specific metabolites and uncover novel metabolic pathways relevant to healthy and diseased mammalian physiology. Group has found that mammalian mitochondria can sustain critical functions in low-oxygen environments.

Mechanistically, they employ fumarate as an alternative electronic scepter for the electron transport chain. This protocol compiles several assays that researchers can use to better assess mitochondrial function in mammalian cells. Importantly, these protocols can be used to study mitochondrial function in hypoxia, as classical respirometry experiments will not be sufficient.

Summary

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Here, we present a compilation of assays to directly measure mitochondrial function in mammalian cells independently of their ability to consume molecular oxygen.

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