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Journal
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Biochemistry
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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
المقايسات المستقلة عن الأكسجين لقياس وظيفة الميتوكوندريا في الثدييات
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Please note that all translations are automatically generated.
Click here for the English version.
المقايسات المستقلة عن الأكسجين لقياس وظيفة الميتوكوندريا في الثدييات
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
DOI:
10.3791/65184-v
•
01:14 min
•
May 19, 2023
•
Tenzin Tseyang*
1
,
Jonathan Valeros*
1
,
Paula Vo*
1
,
Jessica B. Spinelli
2
1
Program in Molecular Medicine
,
University of Massachusetts Chan Medical School
,
2
Cancer Center
,
University of Massachusetts Chan Medical School
Tags
Keywords: Mitochondrial Function
Oxygen-independent Assays
Hypoxia
Metabolite Analysis
Fumarate
Electron Transport Chain
ATP Production
Pyrimidine Biosynthesis
NADH Oxidation
Superoxide Production
Respirometry
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المقايسات المستقلة عن الأكسجين لقياس وظيفة الميتوكوندريا في الثدييات
check_url/65184?article_type=v
13
C
4-الأسبارتات و
13
C
5-الجلوتامين
تتبع النظائر المستقرة ، تحليل LC-MS ، وقياس نشاط SDH
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