Transmitochondrial Cybrid Generation Using Cancer Cell Lines

This research aims to better understand the contribution of mitochondria in cancer progression and metastasis. The transmitochondrial cybrid generation can help us to elucidate how and at what level mitochondrial genotypes are involved in the aggressiveness of a tumor cell. One of the technologies to obtain information on mitochondrial role in cancer is cybrid generation.

We combine this tool with a variety of other methods that include donative electrophoresis, respiration analysis, and other mitochondrial functional assays, flow cytometry, proteomics, et cetera. The plasticity of cancer cells, together with the interaction networks between nuclear and mitochondria genes, increases the complexity of our experiments and data interpretation. This is one of the reasons why it's necessary to develop a new experimental approaches.