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June 02, 2023
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We present a two-step protocol for high-quality mitochondria isolation that is compatible with protein discovery and quantification at a proteome scale. Our protocol does not require genetic engineering and is thus suitable for studying mitochondria from any primary cells and tissues.
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Cite this Article
Blanco-Fernandez, J., Jourdain, A. A. Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification. J. Vis. Exp. (196), e65252, doi:10.3791/65252 (2023).
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