Effective Rapid Blood Perfusion in <em>Xenopus</em>

Rachael A. Jonas-Closs; Leonid Peshkin

doi:10.3791/65287

Journal

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Biology

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Effective Rapid Blood Perfusion in Xenopus

JoVE Journal
Biology

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JoVE Journal Biology

Effective Rapid Blood Perfusion in Xenopus

Effective Rapid Blood Perfusion in Xenopus

4,257 Views

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05:03 min

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May 16, 2023

DOI:

10.3791/65287-v

DOI:

10.3791/65287-v

•

05:03 min

May 16, 2023

•

26 Views

Rachael A. Jonas-Closs¹, Leonid Peshkin¹

¹Department of Systems Biology,Harvard Medical School

Transcript

This procedure centers around the humane utilization of amphibian models in a manner that maximizes efficiency. Standardization practices can be applied to samples taken and preserved at the originating colony, mitigating biosecurity risks and eliminating animal transport stress. Profusion can be completed quickly, maximizing the time available to sample tissues while they are still fresh.

This freshness is especially important for proteomic and transcriptomic profiling. This protocol is detailed and explicit, making it usable for individuals with a wide variety of backgrounds. This facilitates the sharing of high-quality samples between Xenopus labs.

Along with this profusion technique, a similarly comprehensive protocol is being worked on for the standardized sampling of all major organs.

Summary

Presented here is an effective rapid blood perfusion protocol to prepare tissue samples from African clawed frogs for transcriptomics and proteomics studies.