An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry

Hui Ye; Xuemei Liu; JiaBao Liao; Haozhou Huang; Lin Huang; Yang Bao; Hongyan Ma; Junzhi Lin; Xiaoming Bao; Dingkun Zhang; Runchun Xu

doi:10.3791/65291

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An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry

JoVE Journal
Chemistry

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JoVE Journal Chemistry

An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry

1,584 Views

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04:19 min

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March 10, 2023

DOI:

10.3791/65291-v

DOI:

10.3791/65291-v

•

04:19 min

March 10, 2023

•

11 Views

Hui Ye¹, Xuemei Liu¹, JiaBao Liao², Haozhou Huang³, Lin Huang¹, Yang Bao¹, Hongyan Ma¹, Junzhi Lin⁴, Xiaoming Bao⁵, Dingkun Zhang¹, Runchun Xu¹

¹State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy School,Chengdu University of Traditional Chinese Medicine, ²China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co., Ltd, ³Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, ⁴TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine, ⁵Shimadzu (China) Co., Ltd

Transcript

The scope of our research is a material basis in the variation of fish odor in animal medicine. This protocol provides a simple pretreatment method and accurate detection of trimethylamine in animal medicines. The simple pretreatment methods and the programmed parameters of HS-GC mass/mass of this protocol, need to be further optimized, and we still have a lot of work to do.

Our protocol adopts the multi-direction monitoring mode of GC mass/mass, combined with a unique, simple pretreatment, which is not only the first pretreatment for trimethylamine analysis, but also the first analytical mode to be used.

Summary

Here, a headspace gas chromatography-tandem quadrupole mass spectrometry (HS-GC-MS/MS) method suitable for the determination of trimethylamine (TMA) in animal-derived medicines is described. The protocol includes sample pretreatment, headspace treatment, analysis conditions, methodological validation, and the determination of TMA in animal-derived medicines.