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Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry
Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry

Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry

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10:06 min

March 24, 2023

DOI:

10:06 min
March 24, 2023

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Transcript

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We developed polystyrene beads modified with the porphyrin TCPP as compensation controls and multicolor flow cytometry. The beads used in this protocol have fluorescence intensity comparable to TCPP stain cells and still passed through the flow cytometer. We accomplished that and are currently working on better methods to remove the particulate byproduct.

Porphyrin-modified compensation beads were preferable to TCPP stained cells as compensation controls. These speeds were stable over months without significant loss of fluorescence. Porphyrins are known to stain cancer cells selectively.

The research combines the selectivity of porphyrins with quantitative flow cytometry as a powerful platform for high-throughput analysis of human samples.

Summary

Automatically generated

The protocol describes how porphyrin-based compensation beads for flow cytometry are prepared by the reaction of amine-functionalized polystyrene beads with the porphyrin TCPP and the amide coupling reagent EDC. A filtration procedure is used to reduce the particulate byproducts.

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