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Journal
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Biology
/
Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs
/
Medidas em Tempo Real de Parâmetros de Cálcio e Contratilidade em Cardiomiócitos Derivados de Células-Tronco Pluripotentes Induzidos Humanos
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Medidas em Tempo Real de Parâmetros de Cálcio e Contratilidade em Cardiomiócitos Derivados de Células-Tronco Pluripotentes Induzidos Humanos
Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs
DOI:
10.3791/65326-v
•
01:21 min
•
May 26, 2023
•
Rafeeh Dinani
2
,
Emmy Manders
2,3
,
Michiel Helmes
2,3
,
Lili Wang
,
Bjorn C. Knollmann
,
Diederik W. D. Kuster
2
,
Jolanda van der Velden
2
1
Division of Physiology, Amsterdam UMC
,
Vrije University
,
2
Heart Failure & Arrhythmias
,
Amsterdam Cardiovascular Sciences
,
3
CytoCypher BV
,
4
Division of Clinical Pharmacology
,
Vanderbilt School of Medicine
Tags
Keywords: Human Induced Pluripotent Stem Cell-derived Cardiomyocytes
HiPSC-CMs
Contractility
Calcium Handling
Real-time Measurements
Pixel Correlation
Calcium-sensitive Fluorophore
Fura-2
2D Platform
Preclinical Drug Screening
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