Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Stephanie Ibrahim; Catherine Robert; Camille Humbert; Clotilde Ferreira; Gwena&#235;lle Collod; Sonia Stefanovic

doi:10.3791/65328

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Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

2,971 Views

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10:03 min

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May 12, 2023

DOI:

10.3791/65328-v

DOI:

10.3791/65328-v

•

10:03 min

May 12, 2023

•

2938 Views

Stephanie Ibrahim¹, Catherine Robert², Camille Humbert², Clotilde Ferreira¹, Gwenaëlle Collod², Sonia Stefanovic¹

¹Center for Cardiovascular Disease & Nutrition, U1263,Aix-Marseille University ²Marseille Medical Genetics, U1251,Aix-Marseille University

Summary

Here, we present a protocol describing cell nuclei preparation. After microdissection and enzymatic dissociation of cardiac tissue into single cells, the progenitor cells were frozen, followed by isolation of pure viable cells, which were used for single-nucleus RNA sequencing and the single-nucleus assay for transposase-accessible chromatin with high-throughput sequencing analyses.