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Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli
JoVE Journal
Genetics
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JoVE Journal Genetics
Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli

Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli

DOI:
10.3791/65342-v

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15:00 min

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August 18, 2023

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, , , , , ,
1Department of Molecular Biosciences,The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action,Michigan State University, 3Department of Microbiology and Molecular Genetics,Michigan State University, 4Department of Integrative Biology,Michigan State University, 5Biological Sciences Program,Michigan State University

Chapters

  • 00:03Introduction
  • 02:02Daily Transfers of LTEE Populations
  • 06:04Competitive Fitness Assays
  • 06:44Prepare Supplies
  • 08:24Day -2: Revive Competitors
  • 09:09Day -1: Precondition Competitors
  • 09:47Day 0: Begin Competition
  • 11:16Alternative:Continue Three-Day Competition
  • 11:43Day 1 or 3: Finish Competition
  • 12:41Calculate and Plot Fitness
  • 12:52Representative Results Appearance and turbidity of LTEE Cultures
  • 13:22Representative Results Co-culture Competition Results
  • 14:39Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to maintain the Escherichia coli Long-Term Evolution Experiment (LTEE) by performing its daily transfers and periodic freeze-downs and how to conduct competition assays to measure fitness improvements in evolved bacteria. These procedures can serve as a template for researchers starting their own microbial evolution experiments.

Tags

Keywords: Daily TransfersArchivingFitness MeasurementLong-term Evolution ExperimentEscherichia ColiGlucose-limited MediumREL606REL607Ara+Ara-Colony ColorCo-culture Competition AssaysRelative Fitness

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