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JoVE Journal Genetics
Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli
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Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli

Article DOI: 10.3791/65342-v • 15:00 min • August 18th, 2023
August 18th, 2023 •
1,2, 2,3, 2,4, 1, 1, 2,3, 2,5
1Department of Molecular Biosciences, The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action, Michigan State University, 3Department of Microbiology and Molecular Genetics, Michigan State University, 4Department of Integrative Biology, Michigan State University, 5Biological Sciences Program, Michigan State University

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Summary
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This protocol describes how to maintain the Escherichia coli Long-Term Evolution Experiment (LTEE) by performing its daily transfers and periodic freeze-downs and how to conduct competition assays to measure fitness improvements in evolved bacteria. These procedures can serve as a template for researchers starting their own microbial evolution experiments.

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Daily Transfers Archiving Populations Measuring Fitness Long-term Evolution Experiment Escherichia Coli Richard Lenski Erlenmeyer Flasks Glucose-limited Growth Medium DM25 REL606 Strain REL607 Strain Arabinose Utilization Ancestral Strains Tetrazolium Agar Arabinose Agar Shaking Incubator 37 Degrees Celsius Transfer And Regrowth Cycles Long History Of Evolution LTEE Populations Contamination Checking Frozen Record
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