This protocol is designed to identify the presence of Leptospira DNA in water samples. The dot-blot technique is utilized for this purpose, which enables accurate detection and analysis of the DNA. The dot-blot is an easy and versatile non-isotopic technique and may help to answer key questions about the environmental distribution of Leptospira.
This method has the potential to assess huge water bodies and aid in disease surveillance.
Summary
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In this study, a dot-blot application was designed to detect Leptospira from the three main clades in water samples. This method allows for the identification of minimal DNA quantities specifically targeted by a digoxigenin-labeled probe, easily detected by an anti-digoxigenin antibody. This approach is a valuable and satisfactory tool for screening purposes.
Delgadillo-Tellez, E., Zavaleta-Villa, B., Atilano-López, D., Hernández-Castro, R., Olivo-Díaz, A., Carrillo-Casas, E. M. Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples. J. Vis. Exp. (208), e65435, doi:10.3791/65435 (2024).