Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

Michael Hamersky IV; Khushi Tekale; L. Matthew Winfree; Matthew J. M. Rowan; Lindsey Seldin

doi:10.3791/65529

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Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

JoVE Journal
Biology

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JoVE Journal Biology

Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

1,463 Views

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04:11 min

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June 30, 2023

DOI:

10.3791/65529-v

DOI:

10.3791/65529-v

•

04:11 min

June 30, 2023

•

6 Views

Michael Hamersky IV¹, Khushi Tekale¹, L. Matthew Winfree², Matthew J. M. Rowan^1,3, Lindsey Seldin^1,4,5,6

¹Department of Cell Biology,Emory University School of Medicine, ²Independent scholar, ³Center for Neurodegenerative Disease,Emory University School of Medicine, ⁴Department of Dermatology,Emory University School of Medicine, ⁵Winship Cancer Institute,Emory University School of Medicine, ⁶Atlanta Veterans Affairs Medical Center

Transcript

We developed a tool to perform intravital imaging on live mice using an inverted confocal microscope. This enables us to track skin cell dynamics throughout homeostasis and compare cell behaviors during disease onset and progression. Multiphoton microscopy is the primary approach applied to achieve intravital cell tracking.

However, the disadvantages of these microscopes include limited day-to-day imaging versatility, high cost, and technical complexity. Our new tool enables the use of versatile inverted confocal microscopes for in vivo intravital imaging. By modifying the design file, the stage insert dimensions can be customized to fit any make of inverted microscope.

The objective hole can be resized and repositioned to fit any animal model and tissue of choice.