1,318 Views
•
05:27 min
•
June 16, 2023
DOI:
Transcript
Automatically generated
The scope of our research is to provide a detailed RNA FISH protocol that’s providing a reference for scientists who want to perform RNA FISH experiments. Our research has established that RNA FISH can be used to localize lncRNA in human osteosarcoma cells. Our protocol provided a comprehensive experimental procedure and the precautions for RNA FISH using lncRNA-SNHG6 in human osteosarcoma cells as a reference.
Summary
Automatically generated
The present protocol describes a method of RNA fluorescence in situ hybridization to localize the lncRNAs in human osteosarcoma cells.
Related Videos
09:25
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Related Videos
18872 Views
08:18
Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
Related Videos
10638 Views
07:31
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
Related Videos
3509 Views
07:06
Visualizing RNA Localization in Xenopus Oocytes
Related Videos
11550 Views
11:24
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
Related Videos
11726 Views
07:43
Methods to Enable Spatial Transcriptomics of Bone Tissues
Related Videos
2413 Views
17:38
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Related Videos
15588 Views
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
Related Videos
18244 Views
14:53
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
Related Videos
12279 Views
10:57
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
Related Videos
10556 Views
Read Article
Cite this Article
Chang, J., Ma, X., Sun, X., Zhou, C., Zhao, P., Wang, Y., Yang, Y. RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells. J. Vis. Exp. (196), e65545, doi:10.3791/65545 (2023).
Copy