Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Cong Chen; Chunyu Huang; Yaya Wu; Zhihong Li; Shuyi Yu; Xiaoyan Chen; Ruochun Lian; Rong Lin; Lianghui Diao; Yong Zeng; Yuye Li

doi:10.3791/65643

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Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

JoVE Journal
Biology

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JoVE Journal Biology

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

1,096 Views

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07:46 min

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October 13, 2023

DOI:

10.3791/65643-v

DOI:

10.3791/65643-v

•

07:46 min

October 13, 2023

•

7 Views

Cong Chen¹, Chunyu Huang^1,2, Yaya Wu¹, Zhihong Li¹, Shuyi Yu¹, Xiaoyan Chen¹, Ruochun Lian¹, Rong Lin¹, Lianghui Diao^1,2, Yong Zeng^1,2, Yuye Li^1,2

¹Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center,Shenzhen Zhongshan Urology Hospital, ²Shenzhen Jinxin Medical Technology Innovation Center, Co., Ltd.

Transcript

Currently, it is challenging to stabilize the analysis within a certain range. It is also difficult to maintain the quality control of the experiment. Our research can help the patients with abnormal endometrial sensitivity in immune to find the cause of reproductive failure.

The local proportion of immune cells in the endometrial can be stably and repeatedly detected. Our findings may promote the research progress of the endometrial sensitivity evaluation in patients with repeated implantation failure.

Summary

Here, a digital immunohistochemistry image analysis platform was developed and validated to quantitatively analyze the endometrial immune cells of patients with recurrent miscarriages in the window of implantation.