Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Marianna Gabrielle Almeida Galv&#227;o; Brisa Miranda Andrade Santos; Cah&#234; Moreira Aguiar; Adriana Bozzi

doi:10.3791/65722

Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididym...

JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Full Text

1,587 Views

04:53 min

January 12, 2024

DOI: 10.3791/65722-v

Marianna Gabrielle Almeida Galvão¹, Brisa Miranda Andrade Santos¹, Cahê Moreira Aguiar², Adriana Bozzi^1,2

¹Laboratory of Immunobiology, Department of Biological Sciences,State University of Santa Cruz - UESC, ²Department of Medicine,Afya Medical Sciences College

Overview

This study focuses on the extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from the epididymal adipose tissue of Swiss mice. The findings highlight ADSCs' potential as a therapeutic tool, particularly in managing inflammation associated with diseases like Schistosoma mansoni infection.

Key Study Components

Research Area

Adipose-derived mesenchymal stem cells
Inflammation control
Therapeutic applications in infection

Background

High potential of adipose tissue as a source of stem cells
Represents an abundant and accessible source
Defines a protocol for meticulous collection and processing

Methods Used

Isolation and culture of ADSCs
Swiss mice as the biological model
Characterization techniques including staining and morphological assessment

Main Results

Demonstrated ADSCs' multi-lineage differentiation potential
Effective control of granulomatous reactions in Schistosoma mansoni infection
Characterization confirmed morphology and potential of ADSCs

Conclusions

This study provides a reliable protocol for ADSC extraction and characterization
Highlights the therapeutic promise of ADSCs in inflammatory diseases

Frequently Asked Questions

What are adipose-derived stem cells?

Adipose-derived stem cells (ADSCs) are multipotent stem cells found in adipose tissue, capable of differentiating into various cell types.

How were the stem cells extracted in this study?

Stem cells were extracted from the epididymal adipose tissue of Swiss mice following aseptic techniques and specific processing methods.

What diseases could benefit from ADSC therapy?

Potential applications include treatment for inflammatory diseases and tissue regeneration.

What is the significance of this research?

This research fills a gap in the techniques for isolating ADSCs and demonstrates their therapeutic potential in a disease model.

What methodologies were used for characterization?

Techniques included morphological assessments and staining methods to evaluate differentiation potential.

Can ADSCs be used in combination with other therapies?

Yes, the study indicates that ADSCs can be combined with other treatments, such as praziquantel, to improve therapeutic outcomes.

The adipose tissue is an excellent source of mesenchymal stem cells. Here, we bring the step-by-step extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from Swiss mice epididymal adipose tissue.

My research focus on adipose-derived mesenchymal stem cells as a new approach treatment, associated or not associated with other therapies, to improve inflammation from several diseases. We demonstrated that using adipose mesenchymal stem cells in conjunction with praziquantel has significantly controlled the granulomatous reaction in Schistosoma mansoni infection, highlighting the benefits of this combined treatment approach. Our protocol address the research gap in the meticulous collection of epididymal adipose tissue under aseptic conditions, the detailed physical processing involving vigorous shaking, and identifies the optimal time for utilizing adipose-derived stem cells.

Our protocol offers significant advantages, notably the abundant availability and the potential straightforward isolation of mesenchymal stem cells. Additionally, mesenchymal stem cells possess potent therapeutic properties including anti-inflammatory and tissue generation capabilities. To begin, place a euthanized mouse on a corkboard with the ventral side facing up.

View the full transcript and gain access to thousands of scientific videos