Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

Renata Belis&#225;rio; Maria F. Torres; Ester A. S. Buiate; Katia V. Xavier; Etta M. Nuckles; Lisa J. Vaillancourt

doi:10.3791/65755

Journal

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Biology

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Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

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Biology

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JoVE Journal Biology

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

1,334 Views

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06:12 min

•

September 15, 2023

DOI:

10.3791/65755-v

DOI:

10.3791/65755-v

•

06:12 min

September 15, 2023

•

11 Views

Renata Belisário¹, Maria F. Torres^1,2, Ester A. S. Buiate^1,3, Katia V. Xavier^1,4, Etta M. Nuckles¹, Lisa J. Vaillancourt¹

¹Department of Plant Pathology,University of Kentucky, ²Department of Biological Sciences,University of Cincinnati, ³Bayer Crop Science, ⁴Everglades Research and Education Center,University of Florida

Transcript

So our team aims to understand fungal pathogenicity mechanisms in plants to improve disease management. We are currently exploring how the maize autochthonous fungus regulates protein secretion beyond its housekeeping function. Our protocol shows high synchronicity across replications and reproducibility of fungal colonization.

Also, with our assays, there is no need for clearing or fixating samples before imaging them in real-time. The method offers a variety of applications in plant-pathogen interactions and contributed to detailed studies on the mechanisms of fungal pathogenicity in maize and comparative genomic analysis.

Summary

This manuscript details an optimized inoculation protocol that uses detached maize leaf sheaths for reproducible cytological, physiological, and molecular studies of maize interactions with fungal plant pathogens. The leaf sheaths facilitate real-time observation of cellular interactions between the living plant and fungus in unfixed tissues.