Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

Fengchan Li; Zhen Zhu; Yun Du; Li Zhu; Chaojun Tang

doi:10.3791/65863

Journal

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Biology

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Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

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Biology

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JoVE Journal Biology

Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

2,662 Views

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04:21 min

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January 19, 2024

DOI:

10.3791/65863-v

DOI:

10.3791/65863-v

•

04:21 min

January 19, 2024

•

21 Views

Fengchan Li¹, Zhen Zhu¹, Yun Du¹, Li Zhu^1,2,3, Chaojun Tang^1,2,3

¹Cyrus Tang Hematology Center, Cyrus Tang Medical Institute,Soochow University, ²Collaborative Innovation Center of Hematology of Jiangsu Province,Soochow University, ³Suzhou Key Laboratory of Thrombosis and Vascular Biology,Soochow University

Transcript

Low solubility and the larger cell debrief are the main difficulties of vascular tissue dissociation. Our two steps or digestion method is very suitable for carotid tissue dissociation and may be used to prepare single cell suspicions for other vessels with minor modifications, making it very helpful in cardiovascular research. This protocol is suitable for obtaining multiple cell types in the carotid artery, and it can be easily repeated without special equipment.

In the future, we aim to use cell subclasses discovered by single cell ion sequencing for targeted therapy of cardiovascular disease.