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Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing
JoVE Journal
Biology
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JoVE Journal Biology
Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

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04:21 min

January 19, 2024

DOI:

04:21 min
January 19, 2024

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Transcript

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Low solubility and the larger cell debrief are the main difficulties of vascular tissue dissociation. Our two steps or digestion method is very suitable for carotid tissue dissociation and may be used to prepare single cell suspicions for other vessels with minor modifications, making it very helpful in cardiovascular research. This protocol is suitable for obtaining multiple cell types in the carotid artery, and it can be easily repeated without special equipment.

In the future, we aim to use cell subclasses discovered by single cell ion sequencing for targeted therapy of cardiovascular disease.

Summary

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Here we describe a two-step cell digestion protocol for preparing a single-cell suspension of mouse carotid arteries.

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