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June 21, 2024
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This manuscript outlines a detailed video protocol for culturing primary lens epithelial cells (LECs), aiming to improve reproducibility and aid research in cataracts and posterior capsule opacification (PCO). It offers step-by-step instructions on lens dissection, LECs isolation, and validation, serving as a valuable guide, especially for newcomers in the field.
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Cite this Article
Yu, Y., Zhang, J., Wu, H. Optimizing Mouse Primary Lens Epithelial Cell Culture: A Comprehensive Guide to Trypsinization. J. Vis. Exp. (208), e65912, doi:10.3791/65912 (2024).
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