Vacuum-Forced Agroinfiltration for <em>In planta</em> Transformation of Recalcitrant Plants: Cacao as a Case Study

Hilda E. Hern&#225;ndez-Delgado; Jes&#250;s Alejandro Zamora-Brise&#241;o; Luis Joel Figueroa-Y&#225;&#241;ez; Rafael Urrea-L&#243;pez

doi:10.3791/66024

Journal

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Biology

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Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study

Vacuum-Forced Agroinfiltration for <em>In planta</em> Transformation of Recalcitrant Plants: Cacao as a Case Study

JoVE Journal
Biology

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JoVE Journal Biology

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study

2,382 Views

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05:52 min

•

November 17, 2023

DOI:

10.3791/66024-v

DOI:

10.3791/66024-v

•

05:52 min

November 17, 2023

•

2377 Views

Hilda E. Hernández-Delgado¹, Jesús Alejandro Zamora-Briseño², Luis Joel Figueroa-Yáñez³, Rafael Urrea-López¹

¹Unidad de Biotecnología Vegetal,Centro de Investigación Y Asistencia en Tecnología Y Diseño del Estado de Jalisco (CIATEJ)²Red de Estudios Moleculares Avanzados,Instituto de Ecología ³Unidad de Biotecnología Industrial,Centro de Investigación Y Asistencia en Tecnología Y Diseño del Estado de Jalisco (CIATEJ)

Summary

Here, we present the first protocol for localized vacuum infiltration for in vivo studies of the genetic transformation of large-sized plants. Using this methodology, we achieved for the first time the Agrobacterium-mediated in planta transient transformation of cacao.