Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Ana Lu&#237;sa Gra&#231;a; Niklas Kroner-Weigl; Viviana Reyes Alcaraz; Sigrid M&#252;ller-Deubert; Maximilian Rudert; Denitsa Docheva

doi:10.3791/66047

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Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

JoVE Journal
Bioengineering
Author Produced

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JoVE Journal Bioengineering

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

1,326 Views

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03:35 min

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June 21, 2024

DOI:

10.3791/66047-v

DOI:

10.3791/66047-v

•

03:35 min

June 21, 2024

•

1314 Views

Ana Luísa Graça¹, Niklas Kroner-Weigl², Viviana Reyes Alcaraz¹, Sigrid Müller-Deubert¹, Maximilian Rudert³, Denitsa Docheva¹

¹Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg ²Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery,University Hospital Regensburg ³Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg

Summary

Herein, we demonstrate a three-step organoid model (two-dimensional [2D] expansion, 2D stimulation, three-dimensional [3D] maturation) offering a promising tool for tendon fundamental research and a potential scaffold-free method for tendon tissue engineering.