Assessing Cytotoxicity of Metabolites of Typical Triazole Pesticides in Plants

Our protocol describes a new method for assessing the integral cytotoxicity of triazole pesticide metabolites in plants. Plant callus culture is used to generate the metabolites of proteins in plants, followed by toxicological tests using a human cell line to assess the integral cytotoxicity of metabolites. The metabolites of xenobiotics in plants are extremely complex and only a few stands of metabolites can be obtained.

Hence, toxicological debt of metabolites are limited, which hazards the comprehensive toxicity assessment. This protocol proposed a strategy to assess the integral cytotoxicity of metabolites of triazole pesticides in plants. In this protocol, plant carriers and the human cell models are combined to achieve the integral cytotoxicity assessment of metabolites of triazole pesticides in plants.