Name: Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice (Video) | JoVE Journal
Uploaded: 2024-10-18T12:40:00.000+00:00
Duration: 8 min 50 s
Description: Learn duodenal-jejunal bypass surgery for diabetes research using diet-induced obese diabetic mice. This protocol establishes a reliable model to study metabolic improvements and insulin resistance reduction.

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5 minutes

This study was supported by a grant from the Science and Technology Planning Project of Guangdong Province of China (No. 202002020069).

All protocol steps described below follow the guidelines of the Animal Care and Use Committee of the General Hospital of the Southern Theater Command under the approval number 2020112501.
1. General preoperative preparation
NOTE: Thirty 6-week-old male C57BL/6 mice were purchased. Mice were housed in a Specific Pathogen-Free (SPF) laboratory under a 12 h light/dark cycle; temperature, 22 ± 2 °C; and humidity, 55-65%. Mice were given free access to water and fed a 60% kcal fat diet for 6 weeks to induce obesity. An intraperitoneal injection of 40 mg/kg streptozotocin was administered for 5 days to induce diabetes17. Among the thirty mice, twenty-two mice were screened for random blood glucose &gt; 300 mg/dL and randomly assigned to the DJB (n=15) and sham surgery (n=7) groups. Eight mice were excluded from the experiment due to substandard blood glucose.
<ol>
	<li>Fast the mice for 8 h prior to the surgery. Withdraw water 2 h before the surgery.</li>
	<li>Administer 1% sodium pentobarbital solution (6 mL/kg) and buprenorphine (1 mg/kg) intraperitoneally. Touch the toes or tails of the mice with forceps and ensure that the mice do not show any obvious twitching or shaking. Under adequate anesthesia, the mice can breathe freely without supplemental oxygen.</li>
	<li>Place the mice in the supine position on a sterileboardand under a stereomicroscope. Apply eye ointment to the eyes. Use an electric blanket to keep the mice warm throughout the procedure. Use sterile procedures, including surgical gowns, sterile gloves, and autoclaved instruments.</li>
	<li>In sham control mice, make the two incisions respectively in the duodenum 1 cm below the pylorus and in the jejunum 5 cm below the Treitz and then suture the incisions.</li>
</ol>
2. Duodenal jejunal bypass: Surgical Procedure
<ol>
	<li>Apply the depilatory paste from the xiphoid to the abdomen to remove hair from this region. Wipe off the cream and ensure that the skin is clean. Scrub the area three times with alternating scrub of iodine and alcohol solutions.</li>
	<li>Cover the mouse with a sterile drape, leaving the operational area exposed, and make a 2 cm incision from the xiphoid to the abdomen.</li>
	<li>Use an abdominal retractor to expose the abdominal cavity. Push away the abdominal fat using a wet cotton swab, and move the liver to the cephalic side to fully expose the stomach and bowels.</li>
	<li>The stomach and pylorus are below the liver, and the ligament of Treitz is at the distal duodenum. Double ligate the jejunum, 5 cm distal to the ligament of Treitz with a 6-0 silk suture (Figure 1A). Cut the jejunum at the midpoint of the two ligations and suture the jejunal stump with a 10-0 silk suture (Figure 1B). 
	NOTE: Avoid twisting the mesenteric blood vessels.</li>
	<li>Pull the proximal jejunal incision 5 cm along the bowel to the jejunum to create a jejunal-jejunal anastomosis. Align the two bowels horizontally, and then use a 10-0 silk suture to create the lateral anastomosis (Figure 1C). 
	NOTE: During surgery, keep the bowel moist with saline to reduce water loss. This will prevent the bowel from curling up and will make suturing easier.</li>
	<li>Secure both ends of the bowel and cut the incision to equal length (Figure 1D). Suture the second layer of the posterior intestinal wall with a full-thickness continuous suture. 
	NOTE: The incision length is approximately 0.5-0.6 cm, and the needle distance is ~0.5 mm.</li>
	<li>Secure both ends of the bowel and suture the anterior wall of the bowel. Suture the first layer of the anterior wall with a simple continuous suture and the second layer with a horizontal varus suture (Figure 1E-F). 
	NOTE: Swab the intestinal contents with cotton swabs to prevent infection of the abdominal cavity.</li>
	<li>Pull the distal jejunal incision into the duodenum 1 cm below the pylorus to create a duodenal-jejunal anastomosis (Figure 1G). Suture it using the same method as in steps 2.6-2.7 (Figure 1H-K). 
	NOTE: Check the suture at the anastomotic corner to reduce anastomotic leakage.</li>
	<li>Ligate the bowel with the micro forceps and cut the bowel with the micro scissors. Double ligate the duodenum with a 6-0 silk suture, 2 mm from the distal end of the duodenal-jejunal anastomosis. Cut at the midpoint with micro scissors and suture the stump with a 10-0 silk suture (Figure 1L). 
	NOTE: The gastroduodenal vessels branch perpendicular to the head of the pancreas and are adjacent to the pylorus, requiring careful exploration of the vessels in the transverse section. The confluence of the common bile duct and duodenum was located, taking care not to damage the pancreas or the common bile duct.</li>
	<li>Rinse the abdominal cavity with 30 °C saline. Return the bowel to the abdominal cavity. Suture the muscle and the skin separately with a 6-0 silk. Then, disinfect the skin with iodophor.</li>
	<li>After surgery, inject 30 °C saline (30 mL/kg) subcutaneously in the back to prevent dehydration. Inject penicillin (10 mg/kg) intramuscularly to prevent infection.</li>
</ol>
<img alt="Digestive tract dissection, measurement process with ruler, experimental surgical setup, step-by-step." class="xfigimg" src="/files/ftp_upload/66049/66049fig01.jpg" data-altupdatedat="1788304762000" data-alt="Figure 1"> 
Figure 1: Duodenal-jejunal bypass procedure. (A) Location of the jejunum 5 cm distal to the ligament of Treitz. (B) Double ligate the jejunum with 6-0 silk, cut through the middle of the ligature. (C) Jejunal-jejunal anastomosis. (D) Make a 0.5-0.6 cm incision, and suture the posterior wall. (E-F) Complete Jejunal-jejunal anastomosis. (G) Pull the distal jejunal to the duodenum 1 cm below the pylorus. (H) Duodenal-jejunal anastomosis. (I) Cut the duodenal-jejunal anastomosis with a 0.5-0.6 cm incision and suture the posterior wall with a simple continuous suture. (J-K) Complete duodenal-jejunal anastomosis. (L) At 2 mm from the distal to the duodenal-jejunal anastomosis, double ligate the duodenum with 6-0 silk and cut through the middle of the ligature. <a href="https://www.jove.com/files/ftp_upload/66049/66049fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
3. General postoperative care
<ol>
	<li>After surgery, place the mice on an electric blanket to prevent hypothermia. Allow the mice to crawl freely until fully awake before returning to their cages.</li>
	<li>On the postoperative day, restrict the food and water and inject 2 mL of saline subcutaneously into the back of the mice. On the first postoperative day, give 10 mL of 10% glucose and a functional drink (1:1 ratio) without food, and inject 1 mL of saline subcutaneously.</li>
	<li>On the second and third postoperative days, feed the mice with a mixture of 20 mL of 10% glucose and a functional drink. After the fourth day, give the mice pure water and a high-fat diet. Transitional feeding should be performed according to the postoperative recovery status in the following order: solution, semi-liquid, or solid food.</li>
	<li>Postoperative analgesia: inject buprenorphine (0.1 mg/kg) every 12 h from Days 1 to 3, and then once daily until Day 5.</li>
	<li>After surgery, observe the feeding conditions, activity, feces, and wound healing of the mice.</li>
</ol>
<img alt="Duodenal switch diagram, digestive and biliopancreatic limb anatomy, surgical procedure illustration." class="xfigimg" src="/files/ftp_upload/66049/66049fig02.jpg" data-altupdatedat="1788304762000" data-alt="Figure 2"> 
Figure 2: The diagram of DJB surgery. (A) Duodenal-jejunal anastomosis. (B) Jejunal-jejunal anastomosis. (C) Biliopancreatic limb. (D) Digestive limb. (E) Common limb. <a href="https://www.jove.com/files/ftp_upload/66049/66049fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissected worm anatomy with labeled parts (A-E) and scale for measurement; biological study." class="xfigimg" src="/files/ftp_upload/66049/66049fig03.jpg" data-altupdatedat="1788304762000" data-alt="Figure 3"> 
Figure 3: Anatomy of DJB surgery. (A) Duodenal-jejunal anastomosis. (B) Jejunal-jejunal anastomosis. (C) Biliopancreatic limb. (D) Digestive limb. (E) Common limb. <a href="https://www.jove.com/files/ftp_upload/66049/66049fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
4. Postoperative evaluation of metabolic parameters
<ol>
	<li>Body weight measurement
	<ol>
		<li>Fast the mice for 8 h prior to the measurement. Weigh the mice weekly on Monday before and after surgery for 8 weeks.</li>
	</ol>
	</li>
	<li>Food intake measurement
	<ol>
		<li>House one mouse in each cage. Measure the amount of solid food in each cage before and after 24 h. The difference between the two values represents daily food intake.</li>
	</ol>
	</li>
	<li>Random blood glucose measurement
	<ol>
		<li>At 8:00 a.m. on the weekly Monday of measurement, collect a drop of blood from the tip of the mouse tail and apply it to a glucose strip inserted into the glucometer.</li>
	</ol>
	</li>
	<li>Oral glucose tolerance test
	<ol>
		<li>Eight weeks after the DJB surgery, fast the mice for 8 h before the oral glucose tolerance test. Collect a drop of blood from the tip of the mouse tail and place it on a glucose strip inserted into the glucometer. Administer an oral dose of 20% D-glucose (2 g/kg). Measure the blood glucose levels at 0, 15, 30, 60, 90, 120, 150, and 180 mins after gavage.</li>
	</ol>
	</li>
</ol>

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</ol>

The authors declare no conflicts of interest.

In 1953, Varco et al.18 performed the first jejuno-ileal bypass as the beginning of bariatric surgery. Since then, numerous bariatric surgeries have been performed by surgeons. These surgeries have resulted in weight loss and improved metabolic complications4,19,20. Furthermore, in 1967, Mason and Ito21 performed the first Roux-en-Y gastric bypass (RYGB), which can effectively reduce weight and improve metabolic syndrome. Similar to RYGB, DJB is a stomach-preserving deformation of RYGB that divides the small intestine into the biliopancreatic, digestive, and common limbs. DJB surgery provides significantly durable glycemic control without relying on reduced food intake or weight loss22,23. Therefore, DJB has been recommended as a suitable bariatric surgery for type 2 diabetics with low body mass index24.
The development of bariatric surgery is inseparable from the study of animal models, and most researchers have performed DJB in rats25,26. To date, few studies have used a mouse model to study DJB despite its suitability for studying changes in metabolic indicators after intestinal reconstruction27. The paucity of such studies is due to the fact that surgical models of DJB cannot fully replicate human procedures, making it difficult to compare results between different research groups28. Liang et al.29 initially demonstrated the significant therapeutic effect of DJB surgery on obesity and T2DM in db/db mice. The duodenum was double ligated with 5-0 suture, transected 2 mm distal to the pylorus, cut 4-5 cm below the ligament of Treitz, and the distal jejunum was sutured to the pylorus with a single interrupted end-to-end suture of 9-0 silk thread. The opening of the biliopancreatic limb was sutured with 9-0 silk ~4 cm distal to the jejunal anastomosis. Barataud et al. have also successfully established a mouse model of DJB using db/db mice30. Therefore, in our study, the small bowel was transected 5 cm below the ligament of Treitz. A jejunojejunostomy was performed on the distal 5 cm of the small bowel. The distal jejunum and duodenum were then sutured.
As shown in Table 1, the mice were divided into DJB (n=15) and sham surgery (n=7), nine mice survived in the DJB group, with a survival rate of 60.0%. We have also shown the dead cases and percentage of postoperative deaths in each group. As shown in Table 2, to increase the success rate of this technique, we have continuously improved each step. Intestinal anastomotic bleeding is the most common type of postoperative bleeding, and this complication is the leading cause of death. Therefore, the operation must be performed carefully. An intestinal incision was made at the anti-intestinal mesenteric border to keep away from the blood vessels. In our study, the anastomotic leakage was duodenojejunal anastomosis, which may be due to excessive tension in the anastomosis. Anastomotic leakage has been observed in the duodenal intestinal stump. Therefore, the corners of the anastomosis should be carefully checked during surgery, and if the suture is inadequate, the suture can be used for reinforcement. The suture distance should not be excessively long to reduce anastomotic leakage. Anastomotic obstruction was observed in the jejunal-jejunal anastomosis. Due to the unique physiological structure of the intestine in mice, the small bowel was easily twisted. The anastomotic stoma was shorter than expected when the sutures were pulled tight. During the operation, the mesentery should be adequately dissected, and the bowel should be smoothed out as much as possible to provide enough space for the placement. We should also take care of the suture tightness and the size of the anastomosis.
The main limitation of this DJB technique is that it is performed in mice. The use of microscopic equipment is becoming increasingly important in the establishment of DJB mouse models, as it provides a clear surgical field of view31,32,33. After extensive preliminary experiments, we were familiar with the anatomical structures involved in the surgical procedure and were able to skillfully perform microsurgical manipulations with improved suture precision, reduced surgical injury, and significantly shortened surgical time, thereby enabling DJB surgery in mice. However, the skilled operation of microscopic instruments is a gradual process. We analyzed the causes of death in mice in order to improve the surgical procedure, including shortening the surgical and postoperative recovery times, strengthening the postoperative feeding management, and improving the overall survival rate of the mice.
In conclusion, the application of this surgery in mice allows for a better understanding of the mechanisms underlying the powerful metabolic effects of this procedure. We believe that our study provides a solid foundation for further improving the application of DJB in the treatment of diabetes.

Obesity and type 2 diabetes are the most common chronic diseases in the world, and their prevalence is increasing among young individuals1. Bariatric surgery is the most effective treatment for obesity and diabetes, facilitating long-term blood glucose stabilitywhile also improving the complications associated with obesity2,3. There are several types of bariatric surgery, classified by whether they reduce gastric volume or intestinal absorption; these include restrictive, malabsorptive, and combination4,5.
Duodenal-jejunal bypass (DJB) was first developed by Rubino and Marescaux, who demonstrated that type 2 diabetes could be alleviated by connecting the duodenum and jejunum rather than by reducing the gastric volume6,7. DJB preserves the entire stomach and bypasses the entire duodenum and proximal jejunum. The bowel is divided into biliopancreatic, digestive, and common limbs6,8. DJB shares some similarities with bariatric surgeries, including Roux-en-Y gastric bypass (RYGB), mini-gastric bypass, biliopancreatic bypass, duodenal diversion, and DJB plus sleeve gastrectomy9. Compared to RYGB, DJB does not require a gastrointestinal anastomosis, which reduces the operative time and improves the success rate of the procedure. DJB is similar to RYGB in improving glucose metabolism but does not affect body weight10. After DJB surgery, rapid food delivery to the distal small intestine stimulates the secretion of glucagon-like peptide-1 (GLP-1), resulting in improved glucose metabolism11,12.
The use of animal models is essential for understanding the metabolic, cellular, and molecular pathways. Animal models of bariatric surgery have contributed to our understanding of potential mechanisms underlying obesity and diabetes13,14. However, due to the physiological differences between species, it is impossible to perfectly replicate human diseases in animal models15. Among various animal models available for research purposes, the diet-induced obesity (DIO) mouse model most closely resembles human obesity and metabolic syndrome16. Mice were selected for DJB surgery to determine the feasibility of the surgery and to provide techniques for further research. This manuscript provides a comprehensive summary of both technical aspects and experimental details of DJB surgery.

<table><tbody><tr><td>Abdominal retractor</td><td>F.S.T</td><td>17000-03</td><td>Colibri Retractor -3cm,retractor range 1.5cm/3cm long</td></tr><tr><td>1% sodium pentobarbital solution</td><td>Guangzhou Chemical Reagent Factory</td><td>/</td><td>&amp;nbsp;Dissolved 500mg of pentobarbital sodium powder in 50ml of normal saline to obtain 1% pentobarbital sodium solution.</td></tr><tr><td>Benzylpenicillin sodium for Injection</td><td>North China Pharmaceutical Company Ltd.</td><td>F2062121</td><td>Penicillin</td></tr><tr><td>Buprenorphine</td><td>Guangzhou Chemical Reagent Factory</td><td>/</td><td>Analgesia</td></tr><tr><td>Buprenorphine</td><td>Guangzhou Otsuka Pharmaceutical Co., Ltd</td><td>/</td><td>Analgesic</td></tr><tr><td>Citric acid-sodium citrate buffer</td><td>LEAGENE</td><td>R00522</td><td>Buffer solution</td></tr><tr><td>Cotton buds</td><td>HaoZheng Medical</td><td>60220610</td><td>Cotton swabs</td></tr><tr><td>Depilatory paste</td><td>Veet</td><td>AAPR-S222</td><td>Hair removal cream</td></tr><tr><td>Ear tag</td><td>ZEYA</td><td>SUS304</td><td>Ear-mark</td></tr><tr><td>Electric blanket</td><td>ZOSEN</td><td>ZS-CWDRT</td><td>Heat pad</td></tr><tr><td>Electronic scale</td><td>WETTLER TOLEDO</td><td>20060902-6</td><td>Measure the weight</td></tr><tr><td>Enteral nutritional powder</td><td>Abbott Laboratories</td><td>/</td><td>Nutrition powder</td></tr><tr><td>Eye ointment</td><td>Guangzhou Otsuka Pharmaceutical Co., Ltd</td><td>/</td><td>Protect the eyes</td></tr><tr><td>Glucometer</td><td>Roche</td><td>6993788001</td><td>Assess blood glucose</td></tr><tr><td>Graphpad Prism version 9.4.1</td><td>GraphPad Software</td><td>version 9.4.1</td><td>Software for statistical analysis</td></tr><tr><td>High-fat diet (High Fat [60FDC] Purified Rodent Diet)</td><td>Dyets</td><td>112252</td><td>60kcal% High Fat Diet</td></tr><tr><td>Micro Forceps</td><td>Jinzhong Medical</td><td>18-1140</td><td>Micro forceps</td></tr><tr><td>Micro needle holder</td><td>Jinzhong Medical</td><td>EMT-160-Z</td><td>Needle holder</td></tr><tr><td>Micro Scissors</td><td>Jinzhong Medical</td><td>YBC010</td><td>Micro scissors</td></tr><tr><td>Microscope camera</td><td>LAPSUN</td><td>E 2000</td><td>Video</td></tr><tr><td>Ophthalmic scissors</td><td>Jinzhong Medical</td><td>Y00030</td><td>Surgical scissors</td></tr><tr><td>Pentobarbital</td><td>Guangzhou Chemical Reagent Factory</td><td>/</td><td>Narcosis</td></tr><tr><td>Sodium chloride Injection</td><td>Guangzhou Otsuka Pharmaceutical Co., Ltd</td><td>B21L0301</td><td>NaCl 0.9%</td></tr><tr><td>Stereo microscope</td><td>ZEISS</td><td>Stemi 305</td><td>Binocular stereomicroscope</td></tr><tr><td>Streptozotocin</td><td>Sigma</td><td>S110910-1g</td><td>STZ</td></tr><tr><td>Suture line</td><td>LINGQIAO SUTURE</td><td>ZS-LQPMRZ5/0</td><td>Prolene 6/0,Prolene 10/0</td></tr><tr><td>Tissue forceps</td><td>Jinzhong Medical</td><td>H1701</td><td>Surgical forceps</td></tr></tbody></table>

duodenal-jejunal bypass surgery in diet-induced obese diabetic mice

Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice

The prevalence of obesity and type 2 diabetes is a serious global health concern. Obesity is a major pathogenic factor in type 2 diabetes, cardiovascular disease, and some cancers. Bariatric surgery offers a long-term and effective treatment option for both obesity and diabetes. Sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB) are widely recognized as the most popular bariatric surgeries. Additionally, several exploratory bariatric surgeries have demonstrated promising therapeutic effects. Duodenal-jejunal bypass (DJB), specifically tailored for diabetics with low body mass index, has shown beneficial metabolic outcomes. However, its weight-independent metabolic benefits are not fully understood due to limited animal models. In this article, we describe the optimized care protocols and surgical techniques for performing DJB surgery in diet-induced obese (DIO) diabetic mice. Using a mouse model contributes to a better understanding of the nature of changes induced by DJB surgery while facilitating related clinical practice.

General conditions 
The mean operative time for the DJB procedure was 84.5 ± 2.6 min. Fifteen mice underwent DJB surgery, nine mice survived. As shown in Table 1, the majority of deaths occurred during surgery or in the following 7 days. The causes of postoperative death were bleeding (n=2) on postoperative day 1, anastomotic leakage (n=1) on postoperative day 4, anastomotic obstruction (n=2) on postoperative day 7, and unknown cause (n=1) three weeks after surgery.
For 8 weeks prior to this experiment, we performed DJB surgery on 10 mice weekly as a pre-experiment to simplify the operation. As shown in Figure 4, we obtained a trend of the weekly survival rate of the mice during the pre-experiment. We observed a progressive improvement in the survival rate, indicating that intensive surgical training is required to master the DJB technique.
<img alt="Survival rate graph; postoperative progress, weeks vs. percentage, statistical analysis." class="xfigimg" src="/files/ftp_upload/66049/66049fig04.jpg" data-altupdatedat="1788304762000" data-alt="Figure 4"> 
Figure 4: The survival rate of mice seven days after the DJB Surgery. The data show the benefit of the pre-experiment on the survival rate of DJB mice. The data are shown as the survival rate 7 days post-surgery (%). <a href="https://www.jove.com/files/ftp_upload/66049/66049fig04large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Body weight, food intake, and glucose homeostasis 
The mice had similar body weights and food intakes before surgery and 8 weeks after surgery. DJB mice showed no significant weight loss compared to the sham control mice at 8 weeks after surgery (P &gt; 0.05; Figure 5A). Daily food intake was reduced after the 7 days following DJB and sham control surgery and returned to preoperative levels 3 weeks later (P &gt; 0.05; Figure 5B). We have observed improvements in fasting blood glucose levels and glucose tolerance after DJB surgery (P &lt; 0.01; Figure 5C, 5D).
<img alt="Post-surgery analysis graphs: A) Body weight, B) Food intake, C) Random blood glucose, D) Glucose tolerance." class="xfigimg" src="/files/ftp_upload/66049/66049fig05.jpg" data-altupdatedat="1788304762000" data-alt="Figure 5"> 
Figure 5: Effect of DJB on body weight, daily food intake, random blood glucose, and oral glucose tolerance test. The data are shown as the mean ± SD, n=6. ns: not significant; ** p &lt;0.01 (Mann-Whitney and ANOVA tests). <a href="https://www.jove.com/files/ftp_upload/66049/66049fig05large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Duodenal-jejunal bypass (DJB) surgery can improve glucose metabolism and reduce insulin resistance. Here, we present a protocol to establish a stable and reliable mouse model of DJB.

The prevalence of obesity and type 2 diabetes is a serious global health concern. Obesity is a major pathogenic factor in type 2 diabetes, cardiovascular ...

duodenal-jejunal-bypass-surgery-in-diet-induced-obese-diabetic-mice

Research

JoVE Journal

Medicine

Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental tool.

Roux-en-Y Gastric Bypass Operation in Rats

This video describes a bariatric surgery-Roux-en-Y procedure. This involves the cutting of the jejunum- the middle portion of the intestine- to generate two intestinal limbs. The biliary limb is attached to the existing intestinal loop and the alimentary limb is attached to the stomach, creating a gastric bypass. The gastric bypass created here helps to decrease the nutrient uptake and efficiently reduces body weight.

Roux-en-Y Gastric Bypass Procedure: A Bariatric Surgical Procedure for Gastric Resectioning in Murine Model

Bariatric surgery is the most efficient way to reduce body weight and the deadly metabolic complications (diabetes, obesity, and dyslipidemia) frequently associated with morbid obesity. Mouse models of bariatric surgery represent a unique asset for deciphering molecular mechanisms behind the beneficial effects of these surgeries on diabetes, hypertension, and dyslipidemia.

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

This protocol demonstrates murine islet isolation and seeding onto a decellularized scaffold. Scaffold-supported islets were transplanted into the epididymal fat pad of streptozotocin (STZ)-induced diabetic mice. Islets survived at the transplantation site and reversed the hyperglycemic condition.

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

The following describes the performance of vertical sleeve gastrectomy in mice. This is a type of weight-loss surgery that involves removal of approximately 70% of the stomach.

A Murine Model of Vertical Sleeve Gastrectomy

This protocol is for the performance of one-anastomosis gastric bypass (OAGB) on rat. The operator performs a long and tubular-stapled gastric pouch followed by hand-sewn anastomosis. For this model, the operator reproduces the same ratio between biliopancreatic and common limb in humans; therefore, the biliopancreatic limb measures 35 cm.

One-anastomosis Gastric Bypass (OAGB) in Rats

The prevalence of diabetes and obesity is continuously increasing worldwide. The mechanisms between diabetes, obesity and their associated mortality and co-morbidities need to be further investigated. Here, we present a protocol for sleeve gastrectomy (SG) in animals as an uncomplicated preclinical model of bariatric surgery.

Sleeve Gastrectomy in Mice using Surgical Clips

Roux-en-Y gastric bypass (RYGB) is performed to treat obesity and diabetes. However, the mechanisms underlying RYGB's efficacy are not fully understood, and studies are limited by technical difficulty leading to high mortality in animal models. This article provides instructions on how to perform RYGB in rats with high success rates.

A Protocol for Roux-en-Y Gastric Bypass in Rats using Linear Staplers

Single-anastomosis duodeno-ileal bypass (SADI-S) is an emerging bariatric procedure with important metabolic effects. In this article, we present  a reliable and reproducible model of SADI-S in mice.

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ mice.

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

Learn duodenal-jejunal bypass surgery for diabetes research using diet-induced obese diabetic mice. This protocol establishes a reliable model to study metabolic improvements and insulin resistance reduction.

Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice

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