Cell Patterning Using Magnetic-Archimedes Strategy

Xuhao Zhou; Miribani Maitusong; Tanchen Ren; Yan Wu

doi:10.3791/66063

Journal

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Bioengineering

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Cell Patterning Using Magnetic-Archimedes Strategy

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Cell Patterning Using Magnetic-Archimedes Strategy

1,261 Views

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05:09 min

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February 02, 2024

DOI:

10.3791/66063-v

DOI:

10.3791/66063-v

•

05:09 min

February 02, 2024

•

23 Views

Xuhao Zhou^1,2,3, Miribani Maitusong^1,2,3, Tanchen Ren^1,2,3, Yan Wu^1,2,3

¹Department of Cardiology of The Second Affiliated Hospital,Zhejiang University School of Medicine, ²State Key Laboratory of Transvascular Implantation Devices, ³Cardiovascular Key Laboratory of Zhejiang Province

Transcript

Cell patterning is intuitive and powerful technology for in vitro studies. Our research aims to present a versatile, user-friendly, and inexpensive cell pattern method, which could be replicated in most biomedical laboratories. Compared to other methods, our protocol doesn’t require specialized skills and equipment.

Also, it advances parallel to characters of ink-free, label-free, substrate-independent, and the ability for high-throughput patterning. The protocol requires only two necessities:magnets and the gadolinium-based contrast agents, which are both easy to get at low prices. The magnetic-based cell pattern method provides convenient solutions for the first step of a variety of experiments, including cell migration, biomimetic multicellular co-culture, organoid assembly, biomaterial studies, and more.

Summary

This protocol describes an ink-free, label-free, substrate-independent, high-throughput cell patterning method based on the Magnetic Archimedes effect.