This study provides a detailed and standardized protocol for about precaution preparation under gradual operation that’s providing a reference for those in need. Our research ensures that each animal is dosed equally to produce accurate results. Our study aims to maximize the animal protection and improve the reliability of results from studies of about Decoction formulations.
Summary
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The protocol provides a detailed standardization process of decoction formula and gavage technique with Yiqi Jiedu decoction in the osteosarcoma mouse model as an example. It describes animal protection and improves reliability of research data, providing effective strategies for investigating therapeutic efficacy and molecular mechanisms of decoction formulas in vivo.
Zhou, C., Sun, X., Chang, J., Tong, J., Zhao, P., Guo, S., Yang, Y. Gavage Strategy for Decoction Formula of Traditional Chinese Medicine in Osteosarcoma Model Mice. J. Vis. Exp. (208), e66177, doi:10.3791/66177 (2024).