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February 23, 2024
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Our research focuses on protein targets, prediction and validation of small molecular compounds. The experiment used here shows a method of molecular docking combined with similar thermal shift assay to predict and validate the interaction between small molecules and protein targets. These steps of CETSA are complicated and any steps errors during the operation can have a great impact on the results.
Our protocol combines prediction with verification, effectively reducing both time consumption and economic costs. Furthermore, the experimental instruments and materials are easy to opt in. Finally, the CETSA samples are easy to prepare in test, which ensures the producibility of the samples.
Summary
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The experiment used here shows a method of molecular docking combined with cellular thermal shift assay to predict and validate the interaction between small molecules and protein targets.
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Cite this Article
Luo, L., Xiong, J., Tang, Y., Chen, X., Zhang, H. Protein Target Prediction and Validation of Small Molecule Compound. J. Vis. Exp. (204), e66564, doi:10.3791/66564 (2024).
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