Herbs-Partitioned Moxibustion on the Navel in a Rat Model of Primary Dysmenorrhea with Cold Coagulation and Blood Stasis

Research on the traditional Chinese medicine anesthetic method of primary dysmenorrhea. We want to check if herbs-partitioned moxibustion on the navel can relieve the pain of dysmenorrhea rats. Keeping the rats quiet during the experiment is an experimental challenge that we encountered.

We intend to study the reasons behind the chronic pain in dysmenorrhea and would like to explore the anesthetic mechanism of acupuncture and moxibustion. To begin, obtain the adaptive diet-fed female rat that was maintained in a 12-hour light-dark cycle. Insert the pipette gun with 60 microliters of 0.9%saline into the vaginal orifice of the rat.

Pipette up and down three to four times. Suck out the liquid and drop it onto a glass slide. To fix the dried content, add 60 microliters of 95%ethanol.

After eight minutes, rinse and dry the content. Add 0.1%methylene blue staining solution and incubate away from light for 10 minutes. Afterward, rinse and dry the content.

Observe the slide under the microscope and obtain images to screen the rat's estrus cycle. To establish primary dysmenorrhea, inject the rat with estradiol benzoate subcutaneously in the thigh for 10 days. Place the female rat in a refrigerator at 20 degrees Celsius for four hours on the first to fifth day of modeling.

To begin, establish a primary dysmenorrhea rat model using estradiol benzoate injections. Obtain the moxa cones for herbs-partitioned moxibustion or HPM navel therapy and rub the moxa floss in the hand into a spindle shape. Prepare a dough bowl with a hole in the middle.

To prepare the rat pouch, sew a cut cloth together diagonally. Cut out a small opening at the top for breathing and a hole on one side of the pouch for moxibustion. Then sew a pair of cloth ropes of about 10 centimeters in length and 1 centimeter in width at the top and bottom ends of the bag respectively for tethering.

Next, locate the shenque acupoint on the midline of the rat's abdomen. Remove the hair around the shenque acupoint. Place the rat in the rat pouch, keeping the rat's abdomen up, and expose the lower limbs and tail.

Fix the upper and lower parts of the rat's body and the tail on the operating table with tape. Disinfect the shenque and its surrounding skin with 75%ethanol. Place the bowl on the abdomen, aiming its hole at the shenque acupoint, and put 0.3 grams of the traditional Chinese medicine powder on the acupoint.

Then put the moxa cone on the traditional Chinese medicine powder. Light the moxa cone and treat the rat for 30 minutes. To begin, offer the herbs-partitioned moxibustion navel therapy to a rat with primary dysmenorrhea.

Wipe the experimental box with 75%ethanol before each trial. Place the rat in the cage after intraperitoneal injection of oxytocin or 0.9%saline. Record the writhing behavior of the rat for 30 minutes with a video camera.

After anesthetizing, determine the weight of the treated rat. Incise the skin and muscle from the middle of the abdomen to expose the heart. Insert a profusion needle into the heart from the apex position, fix the profusion needle with hemostatic forceps, and make a small cut in the right auricle.

Perfuse 0.9%saline through the left ventricle and descending aorta until the liver and eyes of the rat become lighter and white in color. Finally, using scissors, cut out the uterus and weigh it accurately to calculate the uterine index. The model group of PDM, with cold coagulation and blood stasis, had a significant increase in writhing score compared to the control group, and the treatment group showed a significant decrease in writhing score.

The model group had a significantly reduced writhing latency compared to the control group, and the treatment group showed an increase in writhing latency compared to the model group. The uterine index increased in the model group compared to the control group and decreased in the treatment group compared to the model group.