An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Jorge Ma&#241;es-Garc&#237;a; Leonardo Beccari; Miguel Torres; Oscar  H. Oca&#241;a

doi:10.3791/66754

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

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05:06 min

May 24, 2024

DOI: 10.3791/66754-v

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

This protocol presents a detailed methodological framework for electroporation-based transgenesis of cardiac cells in developing mouse hearts. The video assets provided here will facilitate learning of this versatile technique.

We aim to establish a new methodology that facilitates the overexpression or downregulation of a certain gene to understand heart development. Mouse transgenesis is a great investment of time and effort. Assessing genes one by one is not realistic because of this and also because of the high complexity of the genome.

Hence, there is a need for a rapid method of screening. We have presented a tool available to any researcher to analyze the gain or loss of function of a candidate gene during heart development. The main advantage of our protocol is that it's much quicker and less expensive than mouse transgenesis.

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