Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

We focus on the seafaring the mechanisms, controlling early cell fate decisions during embryonic stem cell differentiation, both in vitro and in mouse models. Complex regulatory networks, sky differentiation during development, and we investigate how the interplay of signaling pathways, transcription factors, and epigenetic regulators promote different sulfates. Studying the dynamic and rapid process of gastrulation in mice is technically challenging due to the small number of cells per embryo, and the limited number of embryos with the desired genotype per litter.

While literature extensively shows the study of single cells using wild-type or fluorescent-activated cell sorted populations of embryos, a comprehensive analysis of embryos with lineage mutations is still limited. Our protocol offers the possibility of generating single-cell omic datasets from gastrulating embryos harboring lineage mutations. It will help scientists with none or limited mouse handling experience or who wanna learn early embryo manipulation or single-cell approaches.