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January 24, 2025
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This protocol describes in vitro cultivation of the syphilis pathogen Treponema pallidum subsp. pallidum in co-culture with mammalian cells. The method is scalable; it can be used to produce large quantities of T. pallidum and to generate clonal cultures.
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Cite this Article
Edmondson, D. G., Norris, S. J., De Lay, B. D. Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete. J. Vis. Exp. (215), e66880, doi:10.3791/66880 (2025).
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