Name: A Mechanical Construction to Enhance the Stability and Safety of Lifting and Thrusting Manipulation of Acupuncture (Video) | JoVE Journal
Uploaded: 2025-01-10T12:20:00.000+00:00
Duration: 7 min 29 s
Description: Discover a 3D-printed cannula design that standardizes acupuncture manipulation using Simi Motion 3D tracking. This method enhances stability and safety for beginners while improving research consistency.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

This work was supported by Budget Project of Shanghai Municipal Education Commission (Grant Number 2021LK099) and the National Natural Science Foundation of China (Grant Number 82174506).

All procedures in the protocol were conducted on commercially available human simulation materials (see Table of Materials) rather than on humans, so no ethical issues were involved in this study. Informed consent was also obtained from all volunteers who participated in the study. The participants in this experiment were 20 students from the College of Acumox and Tuina at Shanghai University of Traditional Chinese Medicine. These students had completed coursework on the acupuncture lifting and thrusting technique as part of the &#34;Science of Acupuncture and Moxibustion&#34;27curriculum. Additionally, they had nearly a year of practical experience in human needling through lessons and hands-on practice. The details of the equipment and software used are listed in the Table of Materials.
1. Fabrication of the control cannula
<ol>
	<li>Prepare the cannula, the needle sleeve, and the adjustable stopper using 3D printing technology.</li>
	<li>Use white resin as the material for 3D printing to ensure a minimum precision of 0.1 mm, which prevents issues with structures not fitting together due to errors. This material is also more cost-effective and allows easier adjustment of the structure.</li>
</ol>
2. Videography
<ol>
	<li>Camera settings
	<ol>
		<li>Place two tripods in front of the operator&#39;s desk at an appropriate height and connect the two motion cameras. Set the angle between the two motion cameras to 60&#176;-120&#176; (Figure 2A).</li>
		<li>Adjust the camera settings as follows: resolution 1280 &#215; 720 pixels, format MP4, full manual mode (M), aperture F1.2, shutter speed 1/1000s, ISO 6400, auto white balance, and optical zoom 0 mm.</li>
	</ol>
	</li>
	<li>Calibration settings
	<ol>
		<li>Place a 3D calibration stand measuring 15 cm &#215; 15 cm &#215; 15 cm on the table (Figure 2B). Ensure it is within the coverage of the two motion cameras.</li>
	</ol>
	</li>
	<li>Tracking marker placement
	<ol>
		<li>Prepare a passive infrared reflective sphere with a diameter of 6.5 mm. Attach it to the fingernail cap of the participant&#39;s right thumb to measure the movement trajectory.</li>
	</ol>
	</li>
	<li>Experimental operation 
	NOTE: The twenty participants were instructed to perform lifting and thrusting manipulations on the human simulation material, including the following techniques: even lifting and thrusting, heavy thrusting with light lifting, and light thrusting with heavy lifting. Each participant completed the three types of manipulations on the human simulation material, both with and without a cannula set to an amplitude of 15 mm. They then repeated the three manipulations using cannulas with amplitudes of 5 mm, 10 mm, and 15 mm. A 30 min interval was provided between each manipulation session to ensure consistency among the participants. Each manipulation was repeated 10 times.
	<ol>
		<li>Perform lifting and thrusting manipulations without cannula
		<ol>
			<li>Even lifting and thrusting: Insert the needle to a depth of 20 mm. Lift the needle up and down at a uniform rate with an amplitude of 15 mm at a frequency of 60 times per minute.</li>
			<li>Heavy thrusting with light lifting: Insert the needle to a depth of 20 mm. Quickly insert the needle to a certain depth, then slowly withdraw it to the superficial layer with an amplitude of 15 mm at a frequency of 60 times per minute.</li>
			<li>Light thrusting with heavy lifting: Insert the needle to a depth of 20 mm. Slowly insert the needle to a certain depth, then rapidly withdraw it to a shallow layer with an amplitude of 15 mm at a frequency of 60 times per minute.</li>
		</ol>
		</li>
		<li>Perform lifting and thrusting manipulations with cannula 
		NOTE: Fabricate three cannulas that are compatible with the needle size. Adjust their amplitudes to 5 mm, 10 mm, and 15 mm by sliding the adjustable stoppers to the appropriate length.
		<ol>
			<li>Manipulate with the cannula with an amplitude of 5 mm
			<ol>
				<li>Even lifting and thrusting: Fix a needle in a needle sleeve. Place the needle sleeve in a cannula with an amplitude of 5 mm. Insert the needle to a depth of 20 mm and lift the cannula up and down at a uniform rate at a frequency of 60 times per minute.</li>
				<li>Heavy thrusting with light lifting: Use the same cannula. Insert the needle to a depth of 20 mm. Quickly insert the needle to the limited depth, then slowly withdraw it to the superficial layer at a frequency of 60 times per minute.</li>
				<li>Light thrusting with heavy lifting: Use the same cannula. Insert the needle to a depth of 20 mm. Slowly insert the needle to the limited depth, then rapidly withdraw it to the superficial layer at a frequency of 60 times per minute.</li>
			</ol>
			</li>
			<li>Manipulate with the cannula with an amplitude of 10 mm
			<ol>
				<li>Even lifting and thrusting: Fix a needle in a needle sleeve. Place the needle sleeve in a cannula with an amplitude of 10 mm. Insert the needle to a depth of 20 mm and lift the cannula up and down at a uniform rate at a frequency of 60 times per minute.</li>
				<li>Heavy thrusting with light lifting: Use the same cannula. Insert the needle to a depth of 20 mm. Quickly insert the needle to the limited depth, then slowly withdraw it to the superficial layer at a frequency of 60 times per minute.</li>
				<li>Light thrusting with heavy lifting: Use the same cannula. Insert the needle to a depth of 20 mm. Slowly insert the needle to the limited depth, then rapidly withdraw it to the superficial layer at a frequency of 60 times per minute.</li>
			</ol>
			</li>
			<li>Manipulate with the cannula with an amplitude of 15 mm
			<ol>
				<li>Even lifting and thrusting: Fix a needle in a needle sleeve. Place the needle sleeve in a cannula with an amplitude of 15 mm. Insert the needle to a depth of 20 mm and lift the cannula up and down at a uniform rate at a frequency of 60 times per minute.</li>
				<li>Heavy thrusting with light lifting: Use the same cannula. Insert the needle to a depth of 20 mm. Quickly insert the needle to the limited depth, then slowly withdraw it to the superficial layer at a frequency of 60 times per minute.</li>
				<li>Light thrusting with heavy lifting: Use the same cannula. Insert the needle to a depth of 20 mm. Slowly insert the needle to the limited depth, then rapidly withdraw it to the superficial layer at a frequency of 60 times per minute.</li>
			</ol>
			</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>
3. Project configuration of the motion capture and analysis software and video analysis
<ol>
	<li>Video export and renaming 
	NOTE: Transfer all video files from the camera to the designated storage disk on the computer. Rename the 3D calibration video files from cameras 1 and 2 to &#34;1.mp4&#34; and &#34;2.mp4,&#34; respectively.
	<ol>
		<li>Video storage
		<ol>
			<li>Save the operation videos to the computer-designated storage disk. Name them using the participants&#39; full initials in the format &#34;xxx-1&#34; and &#34;xxx-2.&#34;</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Reality motion system project configuration (motion capture and analysis software)
	<ol>
		<li>New project: Start the motion capture and analysis software and select New Project. Set the project name in the project tab, then click on Create and Save to store the project on the specified storage disk.</li>
		<li>Specification: Select Specification &#62; Points &#62; Thumb Tip, drag the tracking point from the predefined point box to the used point box, and click on the Close button to continue.</li>
		<li>Adding camera groups: Right-click on the Cameras &#62; Add Camera Group to add a new camera group.</li>
		<li>Select tracking file: Click on the Select File button in the Tracking box.</li>
		<li>Importing operation video: Click on Open Existing File and select the operation video xxx-1 in the popup window. Click on Apply to complete the video import.</li>
		<li>Import calibration video: Click on Select File in the 3D Calibration box to import the corresponding calibration video &#34;1.mp4.&#34;</li>
		<li>Importing other videos: Following the same steps as in step 3.2.5, import the operation video &#34;xxx-2&#34; and its corresponding calibration video &#34;2.mp4.&#34;</li>
	</ol>
	</li>
	<li>Video analysis
	<ol>
		<li>Opening a camera group: Open the camera group, then right-click on 1.mp4 &#62; Properties.</li>
		<li>Perform 3D calibration: Click on the 3D Calibration button in the 3D Calibration box, enter a description, and add 20 points by clicking on the Add Points button 20 times.</li>
		<li>Setting point parameters: Set the name and corresponding X, Y, Z values for each point, then click on Apply according to the calibration parameters.</li>
		<li>Finish calibration: After configuring all the points, click on each endpoint of the calibration video to complete the 3D calibration.</li>
		<li>Calibrate other cameras: Follow steps 3.3.1-3.3.4 to complete the 3D calibration of the other camera.</li>
		<li>Setting up 3D tracking: Right-click on Camera Group &#62; 3D Tracking, select all cameras, and click on the OK button to open the 3D tracking window.</li>
		<li>Apply mode matching tracking: Set Use Pattern Matching Tracking for both cameras. Manually click on the Thumb Tip point in the first frame.</li>
		<li>Start auto tracking: Click on the Auto Search button to begin automatic 3D tracking frame by frame.</li>
		<li>Complete other video tracking: Follow steps 3.3.6-3.3.8 to complete motion tracking for the other videos. 
		NOTE: If the tracking points are lost during auto 3D tracking, select the row where points are lost, right-click, and select Discard Points From Here. Then, click on the points and the Auto Search button again.</li>
	</ol>
	</li>
	<li>Data export
	<ol>
		<li>Creating 3D calculations: Right-click on Camera Group &#62; New 3D Calculation, select All Cameras, and check Update Data Continuously and Store Data Explicitly in the &#34;Create 3D Data&#34; window. Update data and explicitly store data in a file. Click on the OK button to proceed.</li>
		<li>Export settings: Right-click the folder Containing All the Data &#62; Export.</li>
		<li>Exporting data files: Click on the Export button to export a data file with a customized name (*.txt). Export the other data files in the same way.</li>
	</ol>
	</li>
</ol>
4. Data analysis
<ol>
	<li>Summarization of data
	<ol>
		<li>Measure the spatial dispersion by recording the maximum value of the moving range on the X-, Y-, and Z-axes of the passive infrared reflective sphere on the participants&#39; thumbnail cap (Figure 2C).</li>
		<li>Calculate the standard deviation and take the mean value. Store the data in Microsoft Office Excel files and calculate the mean &#177; standard deviation for graphing.</li>
	</ol>
	</li>
	<li>Analysis of data
	<ol>
		<li>Assess the differences between conditions with and without the cannula by conducting independent samples t-tests (for data consistent with normal distribution) or rank sum tests (for data not consistent with normal distribution).</li>
		<li>Then, perform a two-factor, three-level analysis of variance to evaluate the stability of different lifting and inserting amplitudes. Set the alpha level at p &#60; 0.05 and use the Statistical Package for data analysis to carry out all statistical analyses.</li>
	</ol>
	</li>
</ol>

Control Cannula Improves Precision and Stability in Acupuncture Manipulation

<ol><li>Hang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Efficacy+of+frequently-used+acupuncture+methods+for+specific+parts+and+conventional+pharmaceutical+interventions+in+treating+post-stroke+depression+patients%3A+A+network+meta-analysis%5BTitle%5D)+AND+%22Complement+Ther+Clin+Pract%22%5BJournal%5D)">Efficacy of frequently-used acupuncture methods for specific parts and conventional pharmaceutical interventions in treating post-stroke depression patients: A network meta-analysis.</a> Complement Ther Clin Pract. 45, 101471(2021).</li>
<li>Yoon, D. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Graded+brain+fMRI+response+to+somatic+and+visual+acupuncture+stimulation%5BTitle%5D)+AND+%22Cereb+Cortex%22%5BJournal%5D)">Graded brain fMRI response to somatic and visual acupuncture stimulation.</a> Cereb Cortex. 33 (23), 11269-11278 (2023).</li>
<li>Lee, Y. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Visualizing+motion+patterns+in+acupuncture+manipulation%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Visualizing motion patterns in acupuncture manipulation.</a> J Vis Exp. 113, e54213(2016).</li>
<li>Cao, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+regulations+on+cortical+activation+and+functional+connectivity+of+the+dorsolateral+prefrontal+cortex-primary+somatosensory+cortex+elicited+by+acupuncture+with+reinforcing-reducing+manipulation%5BTitle%5D)+AND+%22Front+Hum+Neurosci%22%5BJournal%5D)">The regulations on cortical activation and functional connectivity of the dorsolateral prefrontal cortex-primary somatosensory cortex elicited by acupuncture with reinforcing-reducing manipulation.</a> Front Hum Neurosci. 17, 1159378(2023).</li>
<li>Yoon, D. E., Lee, I. S., Chae, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identifying+dose+components+of+manual+acupuncture+to+determine+the+dose-response+relationship+of+acupuncture+treatment%3A+A+systematic+review%5BTitle%5D)+AND+%22Am+J+Chin+Med%22%5BJournal%5D)">Identifying dose components of manual acupuncture to determine the dose-response relationship of acupuncture treatment: A systematic review.</a> Am J Chin Med. 50 (3), 653-671 (2022).</li>
<li>Zhu, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acupuncture%2C+from+the+ancient+to+the+current%5BTitle%5D)+AND+%22Anatomical+Rec%22%5BJournal%5D)">Acupuncture, from the ancient to the current.</a> Anatomical Rec. 304 (11), 2365-2371 (2021).</li>
<li>Fu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Efficacy+comparison+of+acupuncture+and+balanced+acupuncture+combined+with+TongduZhengji+manipulation+in+the+treatment+of+acute+lumbar+sprain%5BTitle%5D)+AND+%22Am+J+Trans+Res%22%5BJournal%5D)">Efficacy comparison of acupuncture and balanced acupuncture combined with TongduZhengji manipulation in the treatment of acute lumbar sprain.</a> Am J Trans Res. 14 (7), 4628-4637 (2022).</li>
<li>Kelly, R. B., Willis, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acupuncture+for+Pain%5BTitle%5D)+AND+%22Am+Fam+Physician%22%5BJournal%5D)">Acupuncture for Pain.</a> Am Fam Physician. 100 (2), 89-96 (2019).</li>
<li>Lu, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acupuncture+with+reinforcing+and+reducing+twirling+manipulation+inhibits+hippocampal+neuronal+apoptosis+in+spontaneously+hypertensive+rats%5BTitle%5D)+AND+%22Neural+Regen+Res%22%5BJournal%5D)">Acupuncture with reinforcing and reducing twirling manipulation inhibits hippocampal neuronal apoptosis in spontaneously hypertensive rats.</a> Neural Regen Res. 12 (5), 770-778 (2017).</li>
<li>Yin, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mast+cells+and+nerve+signal+conduction+in+acupuncture%5BTitle%5D)+AND+%22Evid+Based+Complement+Alternat+Med%22%5BJournal%5D)">Mast cells and nerve signal conduction in acupuncture.</a> Evid Based Complement Alternat Med. , 3524279(2018).</li>
<li>Yoon, D. E., Lee, I. S., Chae, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+the+acupuncture+manipulation+properties+of+traditional+East+Asian+medicine+and+Western+medical+acupuncture%5BTitle%5D)+AND+%22Integr+Med+Res%22%5BJournal%5D)">Comparison of the acupuncture manipulation properties of traditional East Asian medicine and Western medical acupuncture.</a> Integr Med Res. 11 (4), 100893(2022).</li>
<li>Xu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rearch+statusand+progresson+acupuncture+technique+parameter+quantitation%5BTitle%5D)+AND+%22Zhong+Hua+Zhong+Yi+Yao+Xue+Kan%22%5BJournal%5D)">Rearch statusand progresson acupuncture technique parameter quantitation.</a> Zhong Hua Zhong Yi Yao Xue Kan. 9 (35), 2255-2258 (2017).</li>
<li>Tang, W. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Motion+video-based+quantitative+analysis+of+the+%27lifting-thrusting%27+method%3A+A+comparison+between+teachers+and+students+of+acupuncture%5BTitle%5D)+AND+%22Acupunct+Med%22%5BJournal%5D)">Motion video-based quantitative analysis of the 'lifting-thrusting' method: A comparison between teachers and students of acupuncture.</a> Acupunct Med. 36 (1), 21-28 (2018).</li>
<li>Errington-Evans, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acupuncture+for+anxiety%5BTitle%5D)+AND+%22CNS+Neurosci+Ther%22%5BJournal%5D)">Acupuncture for anxiety.</a> CNS Neurosci Ther. 18 (4), 277-284 (2012).</li>
<li>Seo, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Motion+patterns+in+acupuncture+needle+manipulation%5BTitle%5D)+AND+%22Acupunct+Med%22%5BJournal%5D)">Motion patterns in acupuncture needle manipulation.</a> Acupunct Med. 32 (5), 394-399 (2014).</li>
<li>Lin, S. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Incidence+of+iatrogenic+pneumothorax+following+acupuncture+treatments+in+Taiwan%5BTitle%5D)+AND+%22Acupunct+Med%22%5BJournal%5D)">Incidence of iatrogenic pneumothorax following acupuncture treatments in Taiwan.</a> Acupunct Med. 37 (6), 332-339 (2019).</li>
<li>Chen, H. N., Chang, C. Y., Chen, L. Z., Chang, Y. J., Lin, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+ultrasonography+measurements+to+determine+the+depth+of+the+GB+21+acupoint+to+prevent+pneumothorax%5BTitle%5D)+AND+%22J+Acupunct+Meridian+Stud%22%5BJournal%5D)">Using ultrasonography measurements to determine the depth of the GB 21 acupoint to prevent pneumothorax.</a> J Acupunct Meridian Stud. 11 (6), 355-360 (2018).</li>
<li>Lyu, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Stimulation+parameters+of+manual+acupuncture+and+their+measurement.+evidence-based+complementary+and+alternative+medicine+%3A+eCAM%5BTitle%5D)+AND+%22Evid+Based+Complement+Alternat+Med%22%5BJournal%5D)">Stimulation parameters of manual acupuncture and their measurement. evidence-based complementary and alternative medicine : eCAM.</a> Evid Based Complement Alternat Med. 2019, 1725936(2019).</li>
<li>Cao, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cerebral+responses+to+different+reinforcing-reducing+acupuncture+manipulations%3A+study+protocol+for+a+randomized+crossover+functional+near-infrared+spectroscopy+%28fNIRS%29+trial%5BTitle%5D)+AND+%22Eur+J+Integr+Med%22%5BJournal%5D)">Cerebral responses to different reinforcing-reducing acupuncture manipulations: study protocol for a randomized crossover functional near-infrared spectroscopy (fNIRS) trial.</a> Eur J Integr Med. 53, 102150(2022).</li>
<li>Su, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intervention+of+the+syndrome-position+point+selection+method+on+idiopathic+tinnitus+of+phlegm-fire+stagnation+pattern%3A+A+randomized+controlled+study%5BTitle%5D)+AND+%22J+Healthc+Eng%22%5BJournal%5D)">Intervention of the syndrome-position point selection method on idiopathic tinnitus of phlegm-fire stagnation pattern: A randomized controlled study.</a> J Healthc Eng. 2022, 9664078(2022).</li>
<li>Chen, B., Lin, K., Xu, L., Cao, J., Gao, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+piezoelectric+force+sensing+and+gesture+monitoring-based+technique+for+acupuncture+quantification%5BTitle%5D)+AND+%22IEEE+Sens+J%22%5BJournal%5D)">A piezoelectric force sensing and gesture monitoring-based technique for acupuncture quantification.</a> IEEE Sens J. 21 (23), 26337-26344 (2021).</li>
<li>Wang, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Role+of+acupoint+area+collagen+fibers+in+anti-inflammation+of+acupuncture+lifting+and+thrusting+manipulation.+Evid.+Based+Complement%5BTitle%5D)+AND+%22Alternat+Med%22%5BJournal%5D)">Role of acupoint area collagen fibers in anti-inflammation of acupuncture lifting and thrusting manipulation. Evid. Based Complement.</a> Alternat Med. 2017, 2813437(2017).</li>
<li>Lin, J. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Understandings+of+acupuncture+application+and+mechanisms%5BTitle%5D)+AND+%22Am+J+Transl+Res%22%5BJournal%5D)">Understandings of acupuncture application and mechanisms.</a> Am J Transl Res. 14 (3), 1469-1481 (2022).</li>
<li>Si, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Acupuncture+with+deqi+modulates+the+hemodynamic+response+and+functional+connectivity+of+the+prefrontal-motor+cortical+network%5BTitle%5D)+AND+%22Front+Neurosci%22%5BJournal%5D)">Acupuncture with deqi modulates the hemodynamic response and functional connectivity of the prefrontal-motor cortical network.</a> Front Neurosci. 15, 693623(2021).</li>
<li>Hu, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+the+quickness+and+duration+of+deqi+on+the+analgesic+effect+of+acupuncture+in+primary+dysmenorrhea+patients+with+a+cold+and+dampness+stagnation+pattern%5BTitle%5D)+AND+%22J+Tradit+Chin+Med%22%5BJournal%5D)">Influence of the quickness and duration of deqi on the analgesic effect of acupuncture in primary dysmenorrhea patients with a cold and dampness stagnation pattern.</a> J Tradit Chin Med. 39 (2), 258-266 (2019).</li>
<li>Li, D. P., Zhang, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exploring+theory+of+contemporary+acupuncture+manipulation+and+its+application+characteristics%3A+In+the+perspective+of+acupuncture+technique+in+acupuncture+and+moxibustion%5BTitle%5D)+AND+%22Zhongguo+Zhen+Jiu%22%5BJournal%5D)">Exploring theory of contemporary acupuncture manipulation and its application characteristics: In the perspective of acupuncture technique in acupuncture and moxibustion.</a> Zhongguo Zhen Jiu. 42 (2), 209-214 (2022).</li>
<li>Wang, F., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aWang%2C+F+%22Acupuncture+and+moxibustion+law%22">Acupuncture and moxibustion law</a>. , Shanghai Science and Technology Press. Shanghai. (2013).</li>
<li>Leow, M. Q. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ultrasonography+in+acupuncture%3A+Uses+in+education+and+research%5BTitle%5D)+AND+%22J+Acupunct+Meridian+Stud%22%5BJournal%5D)">Ultrasonography in acupuncture: Uses in education and research.</a> J Acupunct Meridian Stud. 10 (3), 216-219 (2017).</li>
<li>Leow, M. Q. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantifying+needle+motion+during+acupuncture%3A+Implications+for+education+and+future+research%5BTitle%5D)+AND+%22Acupunct+Med%22%5BJournal%5D)">Quantifying needle motion during acupuncture: Implications for education and future research.</a> Acupunct Med. 34 (6), 482-484 (2016).</li>
<li>Zhang, A., Yan, X. K., Liu, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+introduction+to+a+newly-developed+%26%2334%3BAcupuncture+needle+manipulation+training-evaluation+system%26%2334%3B+based+on+optical+motion+capture+technique%5BTitle%5D)+AND+%22Zhen+Ci+Yan+Jiu%22%5BJournal%5D)">An introduction to a newly-developed &#34;Acupuncture needle manipulation training-evaluation system&#34; based on optical motion capture technique.</a> Zhen Ci Yan Jiu. 41 (6), 556-559 (2016).</li>
<li>Yang, P., Sun, X., Ma, Y., Zhang, C., Zhang, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantification+research+on+acupuncture+manipulation+based+on+video+motion+capture%5BTitle%5D)+AND+%22J+Med+Biomech%22%5BJournal%5D)">Quantification research on acupuncture manipulation based on video motion capture.</a> J Med Biomech. 31 (2), 154-159 (2016).</li>
<li>Tang, W. C., Yang, H. Y., Liu, T. Y., Gao, M., Xu, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Motion+video-based+quantitative+analysis+of+the+%27lifting-thrusting%27+method%3A+A+comparison+between+teachers+and+students+of+acupuncture%5BTitle%5D)+AND+%22Acupunct+Med%22%5BJournal%5D)">Motion video-based quantitative analysis of the 'lifting-thrusting' method: A comparison between teachers and students of acupuncture.</a> Acupunct Med. 36 (1), 21-28 (2018).</li>
<li>Liu, T. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+%26%2334%3BAcupuncture+Manipulation+Information+Analyzing+System%26%2334%3B+in+acupuncture+manipulation+education%5BTitle%5D)+AND+%22Zhongguo+Zhen+Jiu%22%5BJournal%5D)">Application of &#34;Acupuncture Manipulation Information Analyzing System&#34; in acupuncture manipulation education.</a> Zhongguo Zhen Jiu. 29 (11), 927-930 (2009).</li>
</ol>

This study innovatively designed a cannula to improve the stability and safety of acupuncture lifting and inserting manipulations and conducted experiments to evaluate its effectiveness. The researchers used 3D modeling for the structural design and white resin as the material for 3D printing. Compared to manufacturing a metal mold, 3D printing technology offers the advantages of lower cost and easier structural adjustments. Additionally, since the disposable needle is positioned sideways in the groove of the needle slee...

Needling manipulation is performed after the needle is inserted into the patient&#39;s skin to either induce a needle sensation known as &#34;DeQi&#34; (which refers to the sensation of meridian qi induction at the acupuncture point) or to adjust the direction and intensity of the needle sensation. As an essential part of acupuncture, different needling techniques produce varying effects1. Needling manipulation is a critical factor that affects the effectiveness of acupuncture treatment2,3. Research has shown that the signals activated by the lifting-thrusting technique are stronger than those induced by other needling methods4.
The therapeutic effect of acupuncture is closely related to the intensity of stimulation5,6,7, which, in turn, depends on the type of needling manipulation used. As a result, the quantitative-effect relationship of acupuncture manipulation is a key area of experimental research8,9,10. Standardization and reproducibility are crucial to ensuring the scientific validity of acupuncture research11. Both the lifting-thrusting and twisting methods require specific frequency and amplitude of operation12,13, and the selection of acupoints is also important for treating diseases14. However, manual acupuncture relies on human operators, making it difficult to maintain consistent frequency and amplitude during needle manipulation15. Additionally, precautions must be taken to avoid complications such as pneumothorax by carefully controlling the depth and direction of needle insertion in certain areas of the body16,17.
Thus, one of the most urgent challenges in the scientific study of acupuncture manipulation is the development of controllers to improve the stability of needling techniques, which is vital for ensuring the safety and standardization of acupuncture practices18.
Lifting-thrusting is one of the most commonly used basic acupuncture techniques. It involves lifting the needle up and thrusting it down after inserting it into the acupoint at a specific depth. The upward movement is called lifting, while the downward movement is known as thrusting. This process is repeated to achieve the desired clinical effect, with the level of stimulation depending on the amplitude and frequency of the lifting and thrusting motions19,20,21,22. Currently, the amplitude of the lifting and thrusting technique is mainly controlled by the practitioner, and its effectiveness is often evaluated based on the sensation of &#34;De Qi&#34; (the feeling of meridian qi induction at the acupuncture point)23,24,25. However, there is no established standard to evaluate the stability and safety of this technique, and the depth of needle insertion is entirely dependent on the practitioner&#39;s skill.
To promote standardization in acupuncture, several new techniques have been developed to replace traditional manual acupuncture, including pulsed electro-acupuncture, ultrasonic acupuncture, microwave acupuncture, laser acupuncture, and extracorporeal shock wave acupuncture26. While these methods help to some extent in standardizing the effects of acupuncture, they cannot fully replace traditional manual acupuncture in clinical practice. Therefore, standardizing the manipulation of manual acupuncture remains essential.
To address the aforementioned issues, this study designed an acupuncture needle cannula that improves the safety and stability of the lifting and thrusting technique. The control cannula used in the study was manufactured using 3D printing technology (see Table of Materials), and the overall structure consists of three components: the cannula, the needle sleeve, and the adjustable stopper, along with disposable acupuncture needles (Figure 1). The cannula, needle sleeve, and adjustable stopper were all produced through 3D printing technology (see Supplementary File 1, Supplementary File 2, and Supplementary File 3).
The cannula offers several advantages: first, the amplitude is controlled by the stopper, which significantly reduces the burden on practitioners; second, the separation of the needle and cannula prevents contamination during acupuncture; and third, the adjustable scale allows for precise control of the needle&#39;s depth and amplitude, enabling free adjustment as needed. The results of this study provide a safe auxiliary tool for experimental research on acupuncture manipulation, which is crucial for advancing the standardization of acupuncture techniques.

<table><tbody><tr><td>Blender</td><td>Blender Institute B.V.</td><td>Blender 4.2.2 LTS</td><td>Blender is the free and open source 3D creation suite. It supports the entirety of the 3D pipeline&amp;mdash;modeling, rigging, animation, simulation, rendering, compositing and motion tracking, even video editing and game creation. Advanced users employ Blender&#039;s API for Python scripting to customize the application and write specialized tools; often these are included in Blender&#039;s future releases. Blender is well suited to individuals and small studios who benefit from its unified pipeline and responsive development process.</td></tr><tr><td>Human simulation materials</td><td>Dongguan Jiangzhao silicon industry Co., LTD</td><td>Acupuncture exercise skin model</td><td>Portable acupuncture practice skin model, simulated skin, with a ductile layer, can better simulate the feeling of acupuncture.</td></tr><tr><td>IBM SPSS Statistics</td><td>IBM</td><td>R26.0.0.0</td><td>The IBM SPSS Statistics software provides advanced statistical analysis for users of all experience levels. Offering a comprehensive suite of capabilities, it delivers flexibility and usability beyond traditional statistical software.</td></tr><tr><td>Prism 9</td><td>GraphPad Software, LLC.</td><td>GraphPad Prism 9.5.0 (525)</td><td>Prism is a software to draw graphs.</td></tr><tr><td>Simi Reality Motion Systems</td><td>Simi Reality Motion Systems GmbH</td><td>Simi Motion 2D/3D</td><td>Simi Motion provides an&amp;nbsp;extensive platform for motion capture and 2D/3D movement analysis.</td></tr></tbody></table>

a mechanical construction to enhance the stability and safety of lifting and thrusting manipulation of acupuncture

A Mechanical Construction to Enhance the Stability and Safety of Lifting and Thrusting Manipulation of Acupuncture

The therapeutic effectiveness of acupuncture relies on both safety and stability, making these factors essential in acupuncture manipulation research. However, manual manipulation introduces unavoidable inaccuracies, which can impact the reliability of research findings. To address this challenge, a unique lifting and thrusting manipulation control cannula was designed in this study, offering flexible adjustment of movement amplitude. The cannula was created using 3D printing technology, and its effectiveness in maintaining stability was verified by recording the acupuncture needle's movement range with optical sensor technology. The study's results show that the control cannula significantly enhances the stability of acupuncture manipulation, reducing human error. This innovation suggests that the cannula could serve as a valuable auxiliary tool for ensuring both the precision and safety of acupuncture-related experimental research. Its adoption could also contribute to the standardization of acupuncture practices, ensuring more consistent and accurate research outcomes, which is essential for future advancements in acupuncture research and clinical applications.

Effect of the cannula on the stability of the lifting and thrusting manipulation 
Graphs were generated based on the data from one operator, as shown in Figure 3, Figure 4, and Figure 5. The horizontal axis in each figure represents time, and the vertical axis represents the position of the tracking point on the operator's thumb tip, recording the motion trail of this point. Two lines of different colors illustra...

This protocol designs a cannula that can be used to control the range of motion for the lifting and thrusting manipulation in acupuncture, thereby improving stability and safety. It can thus serve both the clinical application and scientific research of acupuncture treatment.

The therapeutic effectiveness of acupuncture relies on both safety and stability, making these factors essential in acupuncture manipulation research. ...

a-mechanical-construction-to-enhance-stability-safety-lifting

Research

JoVE Journal

Medicine

Here, we present a protocol for using the Acupuncture Manipulation Education System (AMES) in the training of acupuncture manipulation skills using phantom acupoints.

Visualizing Motion Patterns in Acupuncture Manipulation

This paper introduces the design and implementation of a bespoke robotic manipulator for extra-corporeal ultrasound examination. The system has five degrees of freedom with lightweight joints made by 3D printing and a mechanical clutch for safety management.

Design and Implementation of a Bespoke Robotic Manipulator for Extra-corporeal Ultrasound

This experimental method describes a solution for the kinematic analysis of acupuncture manipulation with three-dimensional finger motion tracking technology.

Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation

Here, we present a protocol for efficient and rapid electroacupuncture (EA) in mice or young rats using a three-dimensional (3D) printed holder. This technique allows simultaneous operation on multiple animals, saving time and increasing experimental efficiency.

Bulk Electroacupuncture Operation for Mice or Young Rats

Here, we present an improved suspension fixation method using a rat jacket to effectively immobilize rats during acupuncture experiments. Furthermore, the structure and application of this fixation apparatus, as well as instructions and evaluation for its utilization in electroacupuncture therapy, is elucidated.

Improved Suspension Fixation Using Rat Jacket in Rat Acupuncture Experiments

Here, we present a protocol to establish a mouse model of diabetic cardiomyopathy and a mouse electroacupuncture fixation device for the use of electroacupuncture in mice, which can fix the mice more gently during treatment.

Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model

We demonstrated that malignant pigmented lesions with increased metabolic activity generate quantifiable amounts of heat and the measurement of the transient thermal response of the skin to a cooling excitation allows quantitative identification of melanoma and other skin cancers (vs. non-proliferative nevi) at an early stage of the disease.

Quantitative Visualization and Detection of Skin Cancer Using Dynamic Thermal Imaging

Amblyopia is a developmental disorder of the visual cortex that is often accompanied by strong suppression of one eye. We present a new technique for measuring and treating interocular suppression in patients with amblyopia that can be deployed using virtual reality goggles or a portable iPod Touch device.

The Measurement and Treatment of Suppression in Amblyopia

We demonstrate the construction of alcohol vapor chambers using readily available materials that simultaneously house 6 mouse cages. We further describe their use in a mouse model of fetal alcohol exposure equivalent to all 3 trimesters of human pregnancy. This paradigm exposes animals during gestation and postnatal days 1-12.

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

An ELISA offering a novel quantitative approach is described. It specifically detects disease-associated α-synuclein (αSD) in a transgenic mouse model (M83) of synucleinopathy using several antibodies against either the Ser129 phosphorylated αS form or the C-terminal part of the protein.

Detection of Disease-associated &#945;-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated &#945;-synuclein

Discover a 3D-printed cannula design that standardizes acupuncture manipulation using Simi Motion 3D tracking. This method enhances stability and safety for beginners while improving research consistency.

A Mechanical Construction to Enhance the Stability and Safety of Lifting and Thrusting Manipulation of Acupuncture

Explore More Videos