Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Christopher B. Cummings; Samuel S. Catania; Eirene M. Q. Ednacot; Austin J. Kinsella-Johnson; Claire E. Meeds; Jack W. Reynolds; Ava E. Sanderson; Rachel A. Johnson; Katharine R. Watts

doi:10.3791/67067

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Researc...

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

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10:17 min

November 15, 2024

DOI: 10.3791/67067-v

Christopher B. Cummings*^1,2, Samuel S. Catania*^1,2, Eirene M. Q. Ednacot^1,2, Austin J. Kinsella-Johnson^1,2, Claire E. Meeds^1,2, Jack W. Reynolds^1,2, Ava E. Sanderson^1,2, Rachel A. Johnson^1,2, Katharine R. Watts^1,2

¹Department of Chemistry and Biochemistry,California Polytechnic State University, ²Center for Applications in Biotechnology,California Polytechnic State University

An adaptable Gibson Assembly molecular cloning module was employed in a course-based undergraduate research experience (CURE) format for molecular biology laboratory course students. Assessment of student learning outcomes showed improved understanding and confidence in molecular cloning after completion of the CURE, and novel plasmids were cloned for natural product biosynthesis research.